A tri-band microstrip-fed slot antenna for WLAN/WiMAX applications is proposed and investigated. Three operating bands are obtained by inserting split ring resonator (SRR) slots for band-rejected design. An electromagnetic (EM) model of this antenna is simulated by Ansoft HFSS. The principle of operation and parametric of the proposed antenna are also provided. The measured -10 dB bandwidth for return loss is from 2.39 GHz - 2.48 GHz, 3 GHz - 3.7 GHz, and 5 GHz - 7 GHz, covering the WLAN (2.4/5.2/5.8) and WiMAX (3.5/5.5) bands. The proposed antenna achieves a stably omni-directional radiation pattern and acceptable gain at all three operating frequency bands.