Cryopreservation of domestic cat (Felis catus) ovarian tissue: Comparison of two vitrification methods

被引：20

作者：

Brito, D. C. C. ^{[1
,2
]}

Domingues, S. F. S. ^{[2
]}

Rodrigues, A. P. R. ^{[3
]}

Maside, C. ^{[3
]}

Lunardi, F. O. ^{[4
]}

Wu, X. ^{[5
]}

Figueiredo, J. R. ^{[3
]}

Pieczarka, J. C. ^{[1
]}

Santos, R. R. ^{[2
]}

机构：

[1] Fed Univ Para, Biol Sci Inst, Lab Cytogenet, Ctr Adv Studies Biodivers CEABIO, Belem, Para, Brazil

[2] Fed Univ Para, Lab Wild Anim Biol & Med, Belem, Para, Brazil

[3] Univ Estadual Ceara, Fac Vet Med, Lab Manipulat Oocytes & Ovarian Preantral Follicl, Fortaleza, Ceara, Brazil

[4] Fertibaby, Ctr Reprod Med, Fortaleza, Ceara, Brazil

[5] Emory Univ, Rollins Sch Publ Hlth, Hubert Dept Global Hlth, Atlanta, GA USA

来源：

THERIOGENOLOGY | 2018年 / 111卷

关键词：

Preantral follicles; Feline; Functionality; Cryopreservation; Open/closed systems; NUCLEAR ANTIGEN PCNA; IN-VITRO CULTURE; PRIMORDIAL FOLLICLES; EXPRESSION; EXPOSURE; VIABILITY; SUCROSE; GROWTH; CORTEX; MORPHOLOGY;

D O I：

10.1016/j.theriogenology.2018.01.015

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We aimed to evaluate the effect of two vitrification methods on the morphology and functionality of vitrified feline preantral follicles. Feline ovarian tissue was vitrified with EG + trehalose combined or not with dimethyl sulphoxide (DMSO), using two different techniques (open or closed systems). Morphology, developmental capacity and mRNA expression of markers for follicle survival and quality were assessed before and after in vitro culture (IVC). Both vitrification and culture media were serum-free. Vitrification of feline ovarian tissue from five adult domestic cats was performed with EG + trehalose combined or not with DMSO. Two systems were used: the open system solid-surface vitrification (SSV) and the closed system ovarian tissue cryosystem (OTC). Histological analysis of follicle integrity showed that the percentages of normal follicles in previously vitrified ovarian fragments decreased after 7 days of in vitro culture (IVC), independently of the protocol used. Although follicular activation was observed by Ki-67 labelling, this was accompanied by extensive follicular degeneration as detected by a 3-4-fold decrease in follicular density. Remarkable follicle activation was observed in the ovarian tissue vitrified using OTC and subjected to IVC, probably due to a higher rate of degeneration of developing follicles. Even with such follicular loss, the results are promising for the combination of EG + DMSO + trehalose in a serum-free medium when applying the SSV method, with this approach resulting in the highest rates of normal developing follicles (19%) after 7 days IVC, together with granulosa cells proliferating at the same rate observed in fresh tissue. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：69 / 77

页数：9

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