Biogenesis of silver nanoparticles using leaf extracts of Asparagus racemosus and Sophora interrupta: structure characterization, antibacterial and anticancer studies

被引：6

作者：

Satyanarayana, Bethu Murali ^{[1
]}

Reddy, Netala Vasudeva ^{[2
]}

Kommula, Siva Kesava Rao ^{[3
]}

Rao, J. Venkateswara ^{[1
]}

机构：

[1] Indian Inst Chem Technol, Appl Biol Div, CSIR, Hyderabad 500007, Telangana, India

[2] Sri Venkateswara Univ, Dept Biotechnol, Tirupati 517502, Andhra Pradesh, India

[3] Dr YSR Hort Univ, Dept Biochem, Venkataramanna Gudem, Andhra Pradesh, India

来源：

SN APPLIED SCIENCES | 2020年 / 2卷 / 11期

关键词：

Silver nanoparticles; Sophora interrupta; Antibacterial activity; Anticancer activity; Apoptotic studies; Wound healing assay; GREEN SYNTHESIS; METAL NANOPARTICLES; ANTIOXIDANT; SHAPE; SIZE;

D O I：

10.1007/s42452-020-03699-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present investigation reports simple and eco-friendly biogenesis of silver nanoparticles (AgNPs) using leaf extracts of Asparagus racemosus and Sophora interrupta. UV-Vis spectra showed absorption peaks at 413 and 420 nm respectively corresponding to A. racemosus (AR-AgNPs) and S. interrupta (SI-AgNPs). TEM, DLS, XRD and TGA studies revealed that biogenic AgNPs are monodispersed, spherical shaped, thermally stable, FCC crystals and 4-15 nm in size. Zeta potential values were found to be -28.9 and -18.6 mV respectively corresponding to SI-AgNPs and AR-AgNPs. SI-AgNPs exhibited strong antibacterial activities against Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis and Micrococcus luteus with inhibition zones of 11.8, 10.5, 9.8 and 9.1 mm respectively. Further SI-AgNPs exhibited significant cytotoxic properties against SKOV3, DU149, PC3 and A549 cells with IC50 values of 120.87, 191.34, 235.06 and 314.27 mu g/mL respectively. SI-AgNPs induce pronounced apoptotic effects on SKOV3 cells including loss of membrane integrity, chromatin condensation (pyknosis) and nuclear fragmentation (karyorrhexis). SI-AgNPs exhibited significant cell migration inhibition by reducing the wound healing of SKOV3 monolayers. Phytosynthesized AgNPs were found to be biocompatible towards normal (CHO) cells. [GRAPHICS] .

引用

页数：15

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