Flexibility in crosstalk between H2B ubiquitination and H3 methylation in vivo

被引：30
作者：
Vlaming, Hanneke [1 ]
van Welsem, Tibor [1 ]
de Graaf, Erik L. [2 ]
Ontoso, David [3 ]
Altelaar, A. F. Maarten [2 ]
San-Segundo, Pedro A. [3 ]
Heck, Albert J. R. [2 ]
van Leeuwen, Fred [1 ]
机构：
[1] Netherlands Canc Inst, Div Gene Regulat, Amsterdam, Netherlands
[2] Univ Utrecht, Netherlands Prote Ctr, Biomol Mass Spectrometry & Prote Grp, Utrecht, Netherlands
[3] Univ Salamanca, CSIC, Inst Biol Func & Genom, E-37008 Salamanca, Spain
来源：
EMBO REPORTS | 2014年 / 15卷 / 10期
关键词：
chromatin; crosstalk; Dot1; histone ubiquitination; Set1; HISTONE H2B; UBIQUITYLATION; CHROMATIN; DOT1; TRIMETHYLATION; MONOUBIQUITINATION; ACTIVATION; DYNAMICS; COMPLEX; COMPASS;
D O I：
10.15252/embr.201438793
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Histone H2B ubiquitination is a dynamic modification that promotes methylation of histone H3K79 and H3K4. This crosstalk is important for the DNA damage response and has been implicated in cancer. Here, we show that in engineered yeast strains, ubiquitins tethered to every nucleosome promote H3K79 and H3K4 methylation from a proximal as well as a more distal site, but only if in a correct orientation. This plasticity indicates that the exact location of the attachment site, the native ubiquitin-lysine linkage and ubiquitination cycles are not critical for trans-histone crosstalk in vivo. The flexibility in crosstalk also indicates that other ubiquitination events may promote H3 methylation.
引用
收藏
页码：1077 / 1084
页数：8
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