Production of L-malate from fumarate by the yeast Dipodascus magnusii

被引：3
|
作者：
Miková, H
Rosenberg, M
Kristofíková, L
Sulo, P
机构：
[1] Slovak Univ Technol Bratislava, Fac Chem Technol, Dept Biochem Technol, Bratislava 81237, Slovakia
[2] Comenius Univ, Dept Mol Biol, Bratislava 84215, Slovakia
来源：
ACTA BIOTECHNOLOGICA | 1999年 / 19卷 / 04期
关键词：
D O I：
10.1002/abio.370190413
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An alternative microbiological method for the production of malate from fumarate is presented. The yeast Dipodascus magnusii was used for this bioconversion. The optimum cell growth temperature was 28 degrees C and the working volume 120 ml. The highest level of fumarase activity during bioconversion was achieved at a pH of 7.5 and a temperature of 37 degrees C. These conditions were determined as optimal. Using sodium fumarate (1 M), the maximum specific productivity of malic acid obtained was 1.72 g/(g(DCW) x h) for intact cells. In the case of ammonium fumarate, it was 2.25 g/(g(DCW) x h).
引用
收藏
页码：357 / 363
页数：7
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