Characterization of seven polymorphic microsatellite loci in the Common Loon (Gavia immer)

被引:8
作者
Mcmillan, AM
Bagley, MJ
Evers, DC
机构
[1] SUNY Coll Buffalo, Biol Dept, Buffalo, NY 14222 USA
[2] US EPA, Cincinnati, OH 45268 USA
[3] BioDivers Res Inst, Gorham, ME 04038 USA
来源
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 02期
关键词
aves; gaviidae; loons; microsatellite; PCR; primer;
D O I
10.1111/j.1471-8286.2004.00649.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We describe polymerase chain reaction (PCR) primers and conditions to amplify seven microsatellite DNA loci isolated from the Common Loon (Gavia immer). The PCR primers were tested on 83 individuals from 10 locations in North America, including breeding, migration stopover, and wintering areas. Between two and seven alleles were observed to segregate at the seven microsatellite loci, with observed heterozygosities ranging from 0.048 to 0.695.
引用
收藏
页码:297 / 299
页数:3
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