Unamplified gene sensing via Cas9 on graphene

被引：21

作者：

Bruch, Richard ^{[1
,2
]}

Urban, Gerald A. ^{[1
,3
]}

Dincer, Can ^{[1
,2
,4
]}

机构：

[1] Univ Freiburg, Dept Microsyst Engn IMTEK, Lab Sensors, Freiburg, Germany

[2] Univ Freiburg, Freiburg Ctr Interact Mat & Bioinspired Technol F, Freiburg, Germany

[3] Univ Freiburg, Freiburg Mat Res Ctr FMF, Freiburg, Germany

[4] Imperial Coll London, Royal Sch Mines, Dept Bioengn, London, England

来源：

NATURE BIOMEDICAL ENGINEERING | 2019年 / 3卷 / 06期

关键词：

FIELD-EFFECT TRANSISTOR;

D O I：

10.1038/s41551-019-0413-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

An electrical biosensor that relies on the binding of target nucleic acid sequences to Cas9 immobilized on a graphene field-effect transistor enables the rapid detection of mutations in purified samples without the need for nucleic acid amplification. © 2019, Springer Nature Limited.

引用

页码：419 / 420

页数：2

共 14 条

[11] Field-deployable viral diagnostics using CRISPR-Cas13 [J].

Myhrvold, Cameron ;

Freije, Catherine A. ;

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Metsky, Hayden C. ;

Durbin, Ann F. ;

Kellner, Max J. ;

Tan, Amanda L. ;

Paul, Lauren M. ;

Parham, Leda A. ;

Garcia, Kimberly F. ;

Barnes, Kayla G. ;

Chak, Bridget ;

Mondini, Adriano ;

Nogueira, Mauricio L. ;

Isern, Sharon ;

Michael, Scott F. ;

Lorenzana, Ivette ;

Yozwiak, Nathan L. ;

MacInnis, Bronwyn L. ;

Bosch, Irene ;

Gehrke, Lee ;

Zhang, Feng ;

Sabeti, Pardis C. .

SCIENCE, 2018, 360 (6387) :444-448

[12] MicroRNA profiling: approaches and considerations [J].

Pritchard, Colin C. ;

Cheng, Heather H. ;

Tewari, Muneesh .

NATURE REVIEWS GENETICS, 2012, 13 (05) :358-369

[13] Graphene Field-Effect Transistor and Its Application for Electronic Sensing [J].

Zhan, Beibei ;

Li, Chen ;

Yang, Jun ;

Jenkins, Gareth ;

Huang, Wei ;

Dong, Xiaochen .

SMALL, 2014, 10 (20) :4042-4065

[14] BIOSENSING CRISPR-powered diagnostics [J].

Zuo, Xiaolei ;

Fan, Chunhai ;

Chen, Hong-Yuan .

NATURE BIOMEDICAL ENGINEERING, 2017, 1 (06)

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