PPARγ activation induces CD36 expression and stimulates foam cell like changes in rVSMCs

被引：62

作者：

Lim, Hyun-Joung

Lee, Seahyoung

Lee, Kuy-Sook

Park, Jin-Hee

Jang, Yangsoo

Lee, Eun Jig

Park, Hyun-Young

机构：

[1] Yonsei Univ, Project Med Sci BK21, Seoul 120749, South Korea

[2] Yonsei Univ, Coll Med, Yonsei Cardiovasc Genome Ctr, Seoul 120749, South Korea

[3] Yonsei Univ, Coll Med, Div Endocrinol, Seoul 120749, South Korea

[4] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA

来源：

PROSTAGLANDINS & OTHER LIPID MEDIATORS | 2006年 / 80卷 / 3-4期

关键词：

PPAR gamma; CD36; foam cell; atherosclerosis; VSMC;

D O I：

10.1016/j.prostaglandins.2006.06.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of the present study was to determine the role of peroxisome proliferator-activated receptor gamma (PPAR gamma) activation in smooth muscle cell (SMC) derived form cell formation. Wild and mutant type PPAR gamma were delivered by adenovirus then activated with troglitazone. The result of Oil Red O staining and FACS analysis showed that PPAR gamma activation induced lipid accumulation in rVSMCs. Furthermore, PPAR gamma activation reduced SMC marker genes such as alpha-actin while induced adipocyte differentiation marker genes and lipid metabolism-related genes as evidenced by RT-PCR and fluorescent immunocytochemistry. All these data demonstrate that PPAR gamma activation can drive foam cell like change in rVSMCs. Our results strongly suggest that PPAR gamma expression induces CD36 expression and adipocyte differentiation gene activation in the process of atherosclerosis and might be one of the crucial events in SMC derived foam cell formation. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：165 / 174

页数：10

共 42 条

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