Origin and compensation of imaging artefacts in localization-based super-resolution microscopy

被引：9
|
作者：
Erdelyi, M. [1 ]
Sinko, J. [1 ]
Kakonyi, R. [1 ]
Kelemen, A. [2 ]
Rees, E. [3 ]
Varga, D. [1 ]
Szabo, G. [1 ]
机构：
[1] Univ Szeged, Dept Opt & Quantum Elect, H-6720 Szeged, Hungary
[2] Univ Szeged, Dept Appl Informat, H-6725 Szeged, Hungary
[3] Univ Cambridge, Dept Chem Engn & Biotechnol, Cambridge CB2 3RA, England
来源：
METHODS | 2015年 / 88卷
基金：
英国工程与自然科学研究理事会;
关键词：
Super-resolution microscopy; Localization microscopy; Imaging artefacts; Localization error; OPTICAL RECONSTRUCTION MICROSCOPY; SINGLE-MOLECULE LOCALIZATION; RESOLUTION LIMIT; SUBDIFFRACTION-RESOLUTION; ALGORITHMS; ACCURACY; BREAKING; FLUOROPHORES; PERFORMANCE; NANOSCOPY;
D O I：
10.1016/j.ymeth.2015.05.025
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Interpretation of high resolution images provided by localization-based microscopy techniques is a challenge due to imaging artefacts that can be categorized by their origin. They can be introduced by the optical system, by the studied sample or by the applied algorithms. Some artefacts can be eliminated via precise calibration procedures, others can be reduced only below a certain value. Images studied both theoretically and experimentally are qualified either by pattern specific metrics or by a more general metric based on fluorescence correlation spectroscopy. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：122 / 132
页数：11
相关论文
共 50 条
  • [1] Novel organic dyes for multicolor localization-based super-resolution microscopy
    Lehmann, Martin
    Lichtner, Gregor
    Klenz, Haider
    Schmoranzer, Jan
    JOURNAL OF BIOPHOTONICS, 2016, 9 (1-2) : 161 - 170
  • [2] A User's Guide to Localization-Based Super-Resolution Fluorescence Imaging
    Dempsey, Graham T.
    DIGITAL MICROSCOPY, 4TH EDITION, 2013, 114 : 561 - 592
  • [3] A Photoactivatable BODIPY Probe for Localization-Based Super-Resolution Cellular Imaging
    Wijesooriya, Chamari S.
    Peterson, Julie A.
    Shrestha, Pradeep
    Gehrmann, Elizabeth J.
    Winter, Arthur H.
    Smith, Emily A.
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (39) : 12685 - 12689
  • [4] Accessing the third dimension in localization-based super-resolution microscopy
    Hajj, Bassam
    El Beheiry, Mohamed
    Izeddin, Ignacio
    Darzacq, Xavier
    Dahan, Maxime
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2014, 16 (31) : 16340 - 16348
  • [5] Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging
    Dempsey, Graham T.
    Vaughan, Joshua C.
    Chen, Kok Hao
    Bates, Mark
    Zhuang, Xiaowei
    NATURE METHODS, 2011, 8 (12) : 1027 - +
  • [6] Localization-based super-resolution imaging meets high-content screening
    Beghin, Anne
    Kechkar, Adel
    Butler, Corey
    Levet, Florian
    Cabillic, Marine
    Rossier, Olivier
    Giannone, Gregory
    Galland, Remi
    Choquet, Daniel
    Sibarita, Jean-Baptiste
    NATURE METHODS, 2017, 14 (12) : 1184 - +
  • [7] Fast and Efficient Molecule Detection in Localization-Based Super-Resolution Microscopy by Parallel Adaptive Histogram Equalization
    Li, Yiming
    Ishitsuka, Yuji
    Hedde, Per Niklas
    Nienhaus, G. Ulrich
    ACS NANO, 2013, 7 (06) : 5207 - 5214
  • [8] The Lateral and Axial Localization Uncertainty in Super-Resolution Light Microscopy
    Rieger, Bernd
    Stallinga, Sjoerd
    CHEMPHYSCHEM, 2014, 15 (04) : 664 - 670
  • [9] Spatiotemporal Isolation Based Super-Resolution Microscopy
    Lu, Yuxian
    Zhao, Pinlong
    Feng, Jiandong
    CHINESE JOURNAL OF CHEMISTRY, 2023, 41 (13): : 1608 - 1623
  • [10] Spectral demixing avoids registration errors and reduces noise in multicolor localization-based super-resolution microscopy
    Lampe, Andre
    Tadeus, Georgi
    Schmoranzer, Jan
    METHODS AND APPLICATIONS IN FLUORESCENCE, 2015, 3 (03):
12345