Transcriptome-wide distribution and function of RNA hydroxymethylcytosine

被引：328

作者：

Delatte, Benjamin ^{[1
]}

Wang, Fei ^{[2
]}

Long Vo Ngoc ^{[3
]}

Collignon, Evelyne ^{[1
]}

Bonvin, Elise ^{[1
]}

Deplus, Rachel ^{[1
]}

Calonne, Emilie ^{[1
]}

Hassabi, Bouchra ^{[1
]}

Putmans, Pascale ^{[1
]}

Awe, Stephan ^{[4
]}

Wetzel, Collin ^{[5
]}

Kreher, Judith ^{[4
]}

Soin, Romuald ^{[3
]}

Creppe, Catherine ^{[1
]}

Limbach, Patrick A. ^{[5
]}

Gueydan, Cyril ^{[3
]}

Kruys, Veronique ^{[3
]}

Brehm, Alexander ^{[4
]}

Minakhina, Svetlana ^{[2
]}

Defrance, Matthieu ^{[1
]}

Steward, Ruth ^{[2
]}

Fuks, Francois ^{[1
]}

机构：

[1] Univ Libre Bruxelles, ULB Canc Res Ctr U CRC, Fac Med, Lab Canc Epigenet, Brussels, Belgium

[2] Rutgers State Univ, Dept Mol Biol & Biochem, Canc Inst New Jersey, Waksman Inst, Piscataway, NJ 08855 USA

[3] Univ Libre Bruxelles, Fac Sci, Lab Mol Biol Gene, Gosselies, Belgium

[4] Univ Marburg, Inst Mol Biol & Tumorforsch, Marburg, Germany

[5] Univ Cincinnati, Dept Chem, Cincinnati, OH USA

来源：

SCIENCE | 2016年 / 351卷 / 6270期

基金：

美国国家科学基金会;

关键词：

DNA; METHYLATION; 5-HYDROXYMETHYLCYTOSINE; 5-METHYLCYTOSINE; DROSOPHILA; REVEALS; CELLS; TET2;

D O I：

10.1126/science.aac5253

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Hydroxymethylcytosine, well described in DNA, occurs also in RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet in Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing hydroxymethylcytosine in the transcripts of many genes, notably in coding sequences, and identify consensus sites for hydroxymethylation. We found that RNA hydroxymethylation can favor mRNA translation. Tet and hydroxymethylated RNA are found to be most abundant in the Drosophila brain, and Tet-deficient fruitflies suffer impaired brain development, accompanied by decreased RNA hydroxymethylation. This study highlights the distribution, localization, and function of cytosine hydroxymethylation and identifies central roles for this modification in Drosophila.

引用

页码：282 / 285

页数：4

共 15 条

[1] TET2 and TET3 regulate GlcNAcylation and H3K4 methylation through OGT and SET1/COMPASS [J].

Deplus, Rachel ;

Delatte, Benjamin ;

Schwinn, Marie K. ;

Defrance, Matthieu ;

Mendez, Jacqui ;

Murphy, Nancy ;

Dawson, Mark A. ;

Volkmar, Michael ;

Putmans, Pascale ;

Calonne, Emilie ;

Shih, Alan H. ;

Levine, Ross L. ;

Bernard, Olivier ;

Mercher, Thomas ;

Solary, Eric ;

Urh, Marjeta ;

Daniels, Danette L. ;

Fuks, Francois .

EMBO JOURNAL, 2013, 32 (05) :645-655

[2] Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq [J].

Dominissini, Dan ;

Moshitch-Moshkovitz, Sharon ;

Schwartz, Schraga ;

Salmon-Divon, Mali ;

Ungar, Lior ;

Osenberg, Sivan ;

Cesarkas, Karen ;

Jacob-Hirsch, Jasmine ;

Amariglio, Ninette ;

Kupiec, Martin ;

Sorek, Rotem ;

Rechavi, Gideon .

NATURE, 2012, 485 (7397) :201-U84

[3] The mysterious presence of a 5-methylcytosine oxidase in the Drosophila genome Possible explanations [J].

Dunwell, Thomas L. ;

McGuffin, Liam J. ;

Dunwell, Jim M. ;

Pfeifer, Gerd P. .

CELL CYCLE, 2013, 12 (21) :3357-3365

[4] Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA [J].

Fu, Lijuan ;

Guerrero, Candace R. ;

Zhong, Na ;

Amato, Nicholas J. ;

Liu, Yunhua ;

Liu, Shuo ;

Cai, Qian ;

Ji, Debin ;

Jin, Seung-Gi ;

Niedernhofer, Laura J. ;

Pfeifer, Gerd P. ;

Xu, Guo-Liang ;

Wang, Yinsheng .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (33) :11582-11585

[5] Tet2 Facilitates the Derepression of Myeloid Target Genes during CEBPα-Induced Transdifferentiation of Pre-B Cells [J].

Kallin, Eric M. ;

Rodriguez-Ubreva, Javier ;

Christensen, Jesper ;

Cimmino, Luisa ;

Aifantis, Iannis ;

Helin, Kristian ;

Ballestar, Esteban ;

Graf, Thomas .

MOLECULAR CELL, 2012, 48 (02) :266-276

[6] TET enzymes, TDG and the dynamics of DNA demethylation [J].

Kohli, Rahul M. ;

Zhang, Yi .

NATURE, 2013, 502 (7472) :472-479

[7] The Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the Brain [J].

Kriaucionis, Skirmantas ;

Heintz, Nathaniel .

SCIENCE, 2009, 324 (5929) :929-930

[8] Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons [J].

Meyer, Kate D. ;

Saletore, Yogesh ;

Zumbo, Paul ;

Elemento, Olivier ;

Mason, Christopher E. ;

Jaffrey, Samie R. .

CELL, 2012, 149 (07) :1635-1646

[9] EFFECT OF LIGHT ON NUCLEOTIDE COMPOSITION OF RIBOSOMAL-RNA OF WHEAT SEEDLINGS [J].

RACZ, I ;

KIRALY, I ;

LASZTITY, D .

PLANTA, 1978, 142 (03) :263-267

[10] Dnmt2-dependent methylomes lack defined DNA methylation patterns [J].

Raddatz, Guenter ;

Guzzardo, Paloma M. ;

Olova, Nelly ;

Fantappie, Marcelo Rosado ;

Rampp, Markus ;

Schaefer, Matthias ;

Reik, Wolf ;

Hannon, Gregory J. ;

Lyko, Frank .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (21) :8627-8631

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