Using Real-time PCR to assess changes in the crude oil degrading microbial community in contaminated seawater mesocosms

被引：32

作者：

Hassanshahian, Mehdi ^{[1
]}

Yakimov, Michail M. ^{[2
]}

Denaro, Renata ^{[2
]}

Genovese, Maria ^{[2
]}

Cappello, Simone ^{[2
]}

机构：

[1] Shahid Bahonar Univ Kerman, Dept Biol, Fac Sci, Kerman, Iran

[2] CNR Messina, Ist Ambiente Marino Costiero, Messina, Italy

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2014年 / 93卷

关键词：

Biodegradation; Crude-oil; Expression; Mesocosm; Quantification; POLYMERASE-CHAIN-REACTION; PERSIAN-GULF; VIBRIO-ANGUILLARUM; BACTERIA; QUANTIFICATION; GENES; MICROORGANISMS; DEGRADATION; SEQUENCES; WATER;

D O I：

10.1016/j.ibiod.2014.06.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The real-time polymerase chain reaction (RT-PCR) was used to follow changes in the proportion of hydrocarbonoclastic bacteria in the marine microbial community in oil polluted mesocosms during bioremediation field trial. Assay for alk-B1 of Alcanivorax borkumensis and alk-BT of Thalassolituus oleivorans were validated and found to be both sensitive and reproducible. Quantification of alk-B1 from A. borkumensis SK2 in mesocosms show that in single bioaugmentation mesocosm (M1) this gene has high quantity in fifth day of sampling but in biostimulating mesocosm (M2) and consortium bioaugmentation mesocosm (M3) the high quantity of this gene was in tenth day of sampling. The comparison between expression of alk-BT and alk-B1 in M3 mesocosm show that alk-B1 copy number was more than alk-BT. The proportion of alk-B1 or alk-BT containing bacteria was positively correlated to the concentration of crude oil in the mesocosms. After the concentration of crude oil in the mesocosms decreased the gene copy number of alkane monooxygenase genes also decreased. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：241 / 248

页数：8

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