Recombination of two porcine circovirus type 2 strains

被引：40

作者：

Lefebvre, David J. ^{[1
]}

Van Doorsselaere, J. ^{[2
]}

Delputte, P. L. ^{[1
]}

Nauwynck, H. J. ^{[1
]}

机构：

[1] Univ Ghent, Virol Lab, Fac Vet Med, B-9820 Merelbeke, Belgium

[2] KATHO Catholic Univ Coll S W Flanders, Dept Hlth Care & Biotechnol, B-8800 Roeselare, Belgium

来源：

ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 05期

关键词：

MULTISYSTEMIC WASTING-SYNDROME; NUCLEOTIDE-SEQUENCE; GENOTYPE DEFINITION; VIRUS; DISEASE; SWINE;

D O I：

10.1007/s00705-009-0379-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pigs can be concurrently infected with different PCV2 strains. In this study, a cell-culture-adapted PCV2 strain, originating from a PMWS-affected pig, was purified by limiting dilution. Three different strains were obtained, and one of them was a perfect mosaic of the other two, with recombination breakpoints in ORF1 and ORF2. Incongruence was observed between phylogenetic trees constructed with the whole genome, ORF1 and ORF2. Amplification of ORF1 and ORF2 from original material, followed by cloning and sequencing, resulted in sequences corresponding to the parental strains, but not with the mosaic strain. These results demonstrate that PCV2 can undergo recombination.

引用

页码：875 / 879

页数：5

共 25 条

[1] Temporal distribution of porcine circovirus 2 genogroups recovered from postweaning multisystemic wasting syndrome-affected and -nonaffected farms in Ireland and Northern Ireland [J].

Allan, Gordon M. ;

McNeilly, Francis ;

McMenamy, Michael ;

McNair, Irene ;

Krakowka, Steven G. ;

Timmusk, Sirje ;

Walls, Dermot ;

Donnelly, Maria ;

Minahin, Donal ;

Ellis, John ;

Wallgren, Per ;

Fossum, Caroline .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2007, 19 (06) :668-673

[2]

Carman S, 2008, CAN J VET RES, V72, P259

[3] Detection of two porcine circovirus type 2 genotypic groups in United States swine herds [J].

Cheung, A. K. ;

Lager, K. M. ;

Kohutyuk, O. I. ;

Vincent, A. L. ;

Henry, S. C. ;

Baker, R. B. ;

Rowland, R. R. ;

Dunham, A. G. .

ARCHIVES OF VIROLOGY, 2007, 152 (05) :1035-1044

[4] Transcriptional analysis of porcine circovirus type 2 [J].

Cheung, AK .

VIROLOGY, 2003, 305 (01) :168-180

[5] Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars [J].

Cságola, A ;

Kecskeméti, S ;

Kardos, G ;

Kiss, I ;

Tuboly, T .

ARCHIVES OF VIROLOGY, 2006, 151 (03) :495-507

[6] Molecular characterization of Porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs [J].

de Boisséson, C ;

Beven, V ;

Bigarré, L ;

Thiéry, R ;

Rose, N ;

Eveno, E ;

Madec, F ;

Jestin, A .

JOURNAL OF GENERAL VIROLOGY, 2004, 85 :293-304

[7] Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case-control study supports a shift in genotypes with time [J].

Dupont, K. ;

Nielsen, E. O. ;

Baekbo, P. ;

Larsen, L. E. .

VETERINARY MICROBIOLOGY, 2008, 128 (1-2) :56-64

[8] A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence [J].

Grau-Roma, L. ;

Crisci, E. ;

Sibila, M. ;

Lopez-Soria, S. ;

Nofrarias, M. ;

Cortey, M. ;

Fraile, L. ;

Olvera, A. ;

Segales, J. .

VETERINARY MICROBIOLOGY, 2008, 128 (1-2) :23-35

[9] Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs [J].

Hamel, AL ;

Lin, LL ;

Nayar, GPS .

JOURNAL OF VIROLOGY, 1998, 72 (06) :5262-5267

[10] Identification of chicken anemia virus putative intergenotype recombinants [J].

He, Cheng-Qiang ;

Ding, Nai-Zheng ;

Fan, Wei ;

Wu, Yan-Hong ;

Lid, Jing-Peng ;

Li, Yun-long .

VIROLOGY, 2007, 366 (01) :1-7

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