Genetic vaccination for production of DNA-designed antibodies specific to Hepadnavirus envelope proteins

被引：7

作者：

Abouzid, Karima ^{[1
]}

Ndeboko, Benedicte ^{[1
]}

Durantel, Sandra ^{[1
]}

Jamard, Catherine ^{[1
]}

Zoulim, Fabien ^{[1
]}

Buronfosse, Thierry ^{[1
]}

Cova, Lucyna ^{[1
]}

机构：

[1] INSERM, Unit 271, F-69003 Lyon, France

来源：

VACCINE | 2006年 / 24卷 / 21期

关键词：

DNA immunization; plasmid vector; duck hepatitis B virus (DHBV); IgY antibodies; envelope proteins;

D O I：

10.1016/j.vaccine.2005.08.085

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We propose a method of avian antibodies production based on DNA immunization of laying ducks with a plasmid encoding specified antigen, followed by egg collection and purification of egg yolk immunoglobulins (IgY). We have validated this approach in the Duck hepatitis B virus (DHBV) model. We report here that following immunization of female ducks with plasmids encoding DHBV envelope proteins, large amounts (at least 50 mg/egg) of specific antibodies can be obtained from eggs of these ducks. Interestingly, the comparison of different plasmid constructs showed the important differences in their efficacy of specific IgY antibodies induction in the sera and eggs of immunized ducks. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：4615 / 4617

页数：3

共 13 条

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