Correlating Infant Fecal Microbiota Composition and Human Milk Oligosaccharide Consumption by Microbiota of 1-Month-Old Breastfed Infants

被引：104

作者：

Borewicz, Klaudyna ^{[1
]}

Gu, Fangjie ^{[2
]}

Saccenti, Edoardo ^{[3
]}

Arts, Ilja C. W. ^{[4
,5
,6
]}

Penders, John ^{[4
,7
,8
]}

Thijs, Carel ^{[4
]}

van Leeuwen, Sander S. ^{[9
,11
]}

Lindner, Cordula ^{[10
]}

Nauta, Arjen ^{[10
]}

van Leusen, Ellen ^{[10
]}

Schols, Henk A. ^{[2
]}

Smidt, Hauke ^{[1
]}

机构：

[1] Wageningen Univ & Res, Lab Microbiol, Stippeneng 4, NL-6708 WE Wageningen, Netherlands

[2] Wageningen Univ & Res, Lab Food Chem, Bornse Weilanden 9, NL-6708 WG Wageningen, Netherlands

[3] Wageningen Univ & Res, Lab Syst & Synthet Biol, Stippeneng 4, NL-6708 WE Wageningen, Netherlands

[4] Maastricht Univ, CAPHRI Care & Publ Hlth Res Inst, Dept Epidemiol, Minderbroedersberg 4-6, NL-6211 LK Maastricht, Netherlands

[5] Maastricht Univ, CARIM Sch Cardiovasc Dis, Dept Epidemiol, Univ Singel 50, NL-6229 ER Maastricht, Netherlands

[6] Maastricht Ctr Syst Biol MaCSBio, Univ Singel 60, NL-6229 ER Maastricht, Netherlands

[7] Maastricht Univ, Med Ctr, Dept Med Microbiol, P Debyelaan 25, NL-6229 HX Maastricht, Netherlands

[8] NUTRIM Sch Nutr Toxicol & Metab, Univ Singel 40, NL-6229 ER Maastricht, Netherlands

[9] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst GBB, Microbial Physiol, Nijenborgh 7, NL-9747 AG Groningen, Netherlands

[10] FrieslandCampina Innovat Ctr, Bronland 20, NL-6708 WH Wageningen, Netherlands

[11] Univ Med Ctr Groningen, Dept Lab Med, Sect Human Nutr & Hlth, Hanzepl 1, NL-9713 GZ Groningen, Netherlands

来源：

MOLECULAR NUTRITION & FOOD RESEARCH | 2019年 / 63卷 / 13期

关键词：

breastfeeding; human milk oligosaccharide; microbial clusters; microbiome; GUT BACTERIA; FECES; FERMENTATION; MOTHER; GROWTH; ATOPY;

D O I：

10.1002/mnfr.201801214

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Scope Understanding the biological functions of human milk oligosaccharides (HMOs) in shaping gastrointestinal (GI) tract microbiota during infancy is of great interest. A link between HMOs in maternal milk and infant fecal microbiota composition is examined and the role of microbiota in degrading HMOs within the GI tract of healthy, breastfed, 1-month-old infants is investigated. Methods and results Maternal breast milk and infant feces are from the KOALA Birth Cohort. HMOs are quantified in milk and infant fecal samples using liquid chromatography-mass spectrometry. Fecal microbiota composition is characterized using Illumina HiSeq 16S rRNA gene amplicon sequencing. The composition is associated with gender, delivery mode, and milk HMOs: Lacto-N-fucopentaose I and 2 '-fucosyllactose. Overall, Bifidobacterium, Bacteroides, Escherichia-Shigella, and Parabacteroides are predominating genera. Three different patterns in infant fecal microbiota structure are detected. GI degradation of HMOs is strongly associated with fecal microbiota composition, and there is a link between utilization of specific HMOs and relative abundance of various phylotypes (operational taxonomic units). Conclusions HMOs in maternal milk are among the important factors shaping GI tract microbiota in 1-month-old breastfed infants. An infant's ability to metabolize different HMOs strongly correlates with fecal microbiota composition and specifically with phylotypes within genera Bifidobacterium, Bacteroides, and Lactobacillus.

引用

页数：13

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