In vivo microsampling to capture the elusive exposome

被引：29

作者：

Bessonneau, Vincent ^{[1
]}

Ings, Jennifer ^{[2
]}

McMaster, Mark ^{[2
]}

Smith, Richard ^{[3
]}

Bragg, Leslie ^{[4
]}

Servos, Mark ^{[4
]}

Pawliszyn, Janusz ^{[1
]}

机构：

[1] Univ Waterloo, Dept Chem, Waterloo, ON, Canada

[2] Environm Canada, Water Sci & Technol Directorate, Burlington, ON, Canada

[3] Univ Waterloo, Mass Spectrometry Facil, Waterloo, ON, Canada

[4] Univ Waterloo, Dept Biol, Waterloo, ON, Canada

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

SOLID-PHASE MICROEXTRACTION; MASS-SPECTROMETRY; METABOLOMICS; ENVIRONMENT; MUSCLE; ACID;

D O I：

10.1038/srep44038

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

can adversely impact biological interpretation of metabolomics data. In this study, we performed in vivo sampling using solid-phase microextraction (SPME) in combination with non-targeted liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) to capture the fish tissue exposome using molecular networking analysis, and the results were contrasted with molecular differences obtained with ex vivo SPME sampling. Based on 494 MS/MS spectra comparisons, we demonstrated that in vivo SPME sampling provided better extraction and stabilization of highly reactive molecules, such as 1-oleoyl-sn-glycero-3-phosphocholine and 1-palmitoleoyl-glycero-3phosphocholine, from fish tissue samples. This sampling approach, that minimizes sample handling and preparation, offers the opportunity to perform longitudinal monitoring of the exposome in biological systems and improve the reliability of exposure-measurement in exposome-wide association studies.

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