ESR evidence on light-induced nitric oxide formation in higher plant chloroplasts

被引：0

作者：

Liu, B ^{[1
]}

Zhang, SX ^{[1
]}

Song, YG ^{[1
]}

Nie, Z ^{[1
]}

Tian, Q ^{[1
]}

Liu, Y ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Ctr Mol Sci, Beijing 100080, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2004年 / 25卷 / 06期

关键词：

nitric oxide; chloroplast; electron transfer; spin-trapping ESR;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Spin-trapping ESR method was used to study a novel NO formation pathway in higher plant chloroplasts with Fe3+ (MGD)(2) as a NO trap. The oxygen evolution complex (OEC) of the chloroplasts was treated with TEMED, and the electron donor DPC was provided to recover the electron transfer chain. Under anaerobic conditions, NO2- was reduced by strongly illuminating chloroplast to form NO. However when kept in dark or when illuminated without DPC, no NO signal could be detected in the reaction system. The results show that the formation of NO is closely related with the fight-induced electron transfer in higher plant chloroplasts.

引用

页码：1139 / 1141

页数：3

共 18 条

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