Characterization of polyphenol oxidase activity in Ataulfo mango

被引：80

作者：

Cheema, Summervir ^{[1
]}

Sommerhalter, Monika ^{[1
]}

机构：

[1] Calif State Univ East Bay, Dept Chem & Biochem, Hayward, CA 94542 USA

来源：

FOOD CHEMISTRY | 2015年 / 171卷

关键词：

Enzyme; Enzyme activity; Polyphenol oxidase; Ataulfo mango; MANGIFERA-INDICA-L; CV ATAULFO; FRUIT; ANTIOXIDANT;

D O I：

10.1016/j.foodchem.2014.09.011

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Crude extracts of Ataulfo exhibited polyphenol oxidase (PPO) activity with pyrogallol, 3-methylcatechol, catechol, gallic acid, and protocatechuic acid. The substrate dependent pH optima ranged from pH 5.4 to 6.4 with Michaelis-Menten constants between 0.84 +/- 0.09 and 4.6 +/- 0.7 mM measured in MES or phosphate buffers. The use of acetate buffers resulted in larger Michaelis-Menten constants, up to 14.62 +/- 2.03 mM. Sodium ascorbate, glutathione, and kojic acid are promising inhibitors to prevent enzymatic browning in Ataulfo. PPO activity increased with ripeness and was always higher in the skin compared to the pulp. Sodium dodecyl sulphate (SDS) enhanced PPO activity, with pulp showing a stronger increase than skin. SOS-PAGE gels stained for catecholase activity showed multiple bands, with the most prominent bands at apparent molecular weights of 53, 112, and 144 kDa. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：382 / 387

页数：6

共 30 条

[1] BETA-GALACTOSIDASE AND ITS SIGNIFICANCE IN RIPENING MANGO FRUIT [J].

ALI, ZM ;

ARMUGAM, S ;

LAZAN, H .

PHYTOCHEMISTRY, 1995, 38 (05) :1109-1114

[2]

Arogba SS, 1998, J SCI FOOD AGR, V77, P459, DOI 10.1002/(SICI)1097-0010(199808)77:4<459::AID-JSFA61>3.3.CO

[3]

2-F

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] SYSTEMIN ACTIVATES SYNTHESIS OF WOUND-INDUCIBLE TOMATO LEAF POLYPHENOL OXIDASE VIA THE OCTADECANOID DEFENSE SIGNALING PATHWAY [J].

CONSTABEL, CP ;

BERGEY, DR ;

RYAN, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (02) :407-411

[6]

FDA, 2014, ALPH LIST SCOGS SUBS

[7]

Saúco VG, 2013, ACTA HORTIC, V992, P37

[8] Molecular biology of fruit maturation and ripening [J].

Giovannoni, J .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2001, 52 :725-749

[9]

Hanemann P., 2008, 109 USAID

[10] Antioxidant phytochemical and fruit quality changes in mango (Mangifera indica L.) following hot water immersion and controlled atmosphere storage [J].

Kim, Youngmok ;

Brecht, Jeffrey K. ;

Talcott, Stephen T. .

FOOD CHEMISTRY, 2007, 105 (04) :1327-1334

← 1 2 3 →