Overexpression of CDSP32 (GhTRX134) Cotton Gene Enhances Drought, Salt, and Oxidative Stress Tolerance in Arabidopsis

被引：24

作者：

Elasad, Mohammed ^{[1
]}

Ahmad, Adeel ^{[2
]}

Wang, Hantao ^{[2
]}

Ma, Liang ^{[2
]}

Yu, Shuxun ^{[2
]}

Wei, Hengling ^{[2
]}

机构：

[1] Agr Res Corp, POB 126, Wad Madani, Sudan

[2] Chinese Acad Agr Sci, State Key Lab Cotton Biol, Inst Cotton Res, Anyang 455000, Henan, Peoples R China

来源：

PLANTS-BASEL | 2020年 / 9卷 / 10期

关键词：

thioredoxins; abiotic stress; gene cloning; Arabidopsis; transformation; FUNCTIONAL-ANALYSIS; THIOREDOXIN; EXPRESSION; REDUCTASE; PROTEIN; REDOX; TRANSFORMATION; PEROXIREDOXIN; PEROXIDE; FAMILY;

D O I：

10.3390/plants9101388

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Upland cotton (Gossypium hirsutum L.) is the main natural fiber crop worldwide and is an essential source of seed oil and biofuel products. Many abiotic stresses, such as drought and salinity, constrain cotton production. Thioredoxins (TRXs) are a group of small ubiquitous proteins that are widely distributed among organisms. TRXs play a crucial role in regulating diverse functions during plant growth and development. In the present study, a novel GhTRX134 gene was characterized and overexpressed in Arabidopsis and silenced in cotton under drought stress. Furthermore, the proline content and enzyme activity levels were measured in transgenic plants and wild-type (Wt) plants under drought and salt stress. The results revealed that the overexpression of GhTRX134 enhanced abiotic stress tolerance. When GhTRX134 was silenced, cotton plants become more sensitive to drought. Taken together, these findings confirmed that the overexpression of GhTRX134 improved drought and salt tolerance in Arabidopsis plants. Therefore, the GhTRX134 gene can be transformed into cotton plants to obtain transgenic lines for more functional details.

引用

页码：1 / 16

页数：16

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[21] Expression of a bacterial serine acetyltransferase in transgenic potato plants leads to increased levels of cysteine and glutathione [J].