Self-organization of yeast cells on modified polymer surfaces after dewetting: new perspectives in cellular patterning

In recent years, biological micro-electro-mechanical systems ( commonly referred to as BioMEMS) have found widespread use, becoming increasingly prevalent in diagnostics and therapeutics. Cell-based sensors are nowadays gaining increasing attention, due to cellular built-in natural selectivity and physiologically relevant response to biologically active chemicals. On the other hand, surrogate microbial systems, including yeast models, have become a useful alternative to animal and mammalian cell systems for high-throughput screening for the identification of new pharmacological agents. A main obstacle in biosensor device fabrication is the need for localized geometric confinement of cells, without losing cell viability and sensing capability. Here we illustrate a new approach for cellular patterning using dewetting processes to control cell adhesion and spatial confinement on modified surfaces. By the control of simple system parameters, a rich variety of morphologies, ranging through hexagonal arrays, polygonal networks, bicontinuous structures, and elongated fingers, can be obtained.