Genetic Polymorphisms in Chronic Hyperplastic Sinusitis with Nasal Polyposis

被引:38
作者
Bernstein, Joel M. [1 ,2 ,3 ]
Anon, Jack B. [4 ]
Rontal, Michael [5 ]
Conroy, Jeffrey [6 ]
Wang, Chong [7 ]
Sucheston, Lara [7 ,8 ]
机构
[1] SUNY Buffalo, Dept Otolaryngol, Sch Med & Biomed Sci, Buffalo, NY 14260 USA
[2] SUNY Buffalo, Dept Pediat, Sch Med & Biomed Sci, Buffalo, NY 14260 USA
[3] SUNY Buffalo, Dept Communicat Disorders & Sci, Buffalo, NY 14260 USA
[4] Univ Pittsburgh, Dept Otolaryngol, Pittsburgh, PA 15260 USA
[5] Univ Michigan, Dept Otolaryngol, Ann Arbor, MI 48109 USA
[6] Roswell Pk Canc Inst, Micro Array & Genom Facil, Buffalo, NY 14263 USA
[7] SUNY Buffalo, Dept Biostat, Buffalo, NY 14260 USA
[8] Roswell Pk Canc Inst, Div Canc Prevent & Control, Buffalo, NY 14263 USA
关键词
Nasal polyps; genes; single-nucleotide polymorphism; tumor necrosis factor-alpha-308; chronic inflammation; FACTOR-ALPHA PROMOTER; CHRONIC RHINOSINUSITIS; INFLAMMATORY RESPONSE; TNF; EXPRESSION; TRANSCRIPTION; FIBROBLASTS; SECRETION; DISEASE; VCAM-1;
D O I
10.1002/lary.20239
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objectives/Hypothesis: Although many proinflammatory cytokines have been identified in nasal polyp tissue, the initial trigger that causes this inflammation characterized by edema, lymphocytosis, and eosinophilia, is still unknown. The purpose of the present study is to identify the presence of genetic polymorphisms in proinflammatory, anti-inflammatory, and chemokine genes that might contribute to; genetic susceptibility to chronic hyperplastic sinusitis with nasal polyposis (CHSwNP). Study Design: Case control study. Methods: Buccal swabs were taken from the left and right oral mucosal surfaces from 179 patients with CHSwNP and 153 nonpolyposis controls with the Purgene DNA purification protocol (Gentra). Genotyping assays for cytokine gene loci were performed on 14 cytokine genes using the iPlex Gold and the Mass Array Compact system (Sequenom, San Diego, CA). Tests of Hardy-Weinberg equilibrium proportions were performed separately in the cases and controls. Tests for evidence of association between alleles at each single-nucleotide polymorphism (SNP) and case-control status were performed using unconditional logistic regression. Results: The frequency of the A allele in a SNP. located in tumor necrosis factor (TNF)-alpha (rs1800629) is significantly different in patients with nasal polyposis versus controls without nasal polyposis, 18.6%. and 11.5%, respectively with an individuals' odds of susceptibility to nasal polyps increasing almost twofold (odds ratio, 1.86; confidence interval, 1.4-3.09) given at least one copy of the A allele at this SNP All other cytokine gene polymorphisms of both inflammatory, anti-inflammatory, and chemokine genes were not statistically different between the two groups. Conclusions: TNF-alpha-308, a SNP in the promoter region of this cytokine gene is associated with increased odds of developing nasal polyposis. TNF-alpha is a potent immuno-mediator and proinflammatory cytokine that has been implicated in the pathogenesis of a large number of human diseases. The location of this gene on the short arm of chromosome 6, with the major histocompatibility complex genes and complement, has raised the probability that polymorphism within this locus may contribute to a genetic association of this region of the genome with a wide variety of infectious and autoimmune diseases.
引用
收藏
页码:1258 / 1264
页数:7
相关论文
共 33 条
[1]   TESTS FOR LINEAR TRENDS IN PROPORTIONS AND FREQUENCIES [J].
ARMITAGE, P .
BIOMETRICS, 1955, 11 (03) :375-386
[2]   The molecular biology of nasal polyposis [J].
Bernstein J.M. .
Current Allergy and Asthma Reports, 2001, 1 (3) :262-267
[4]   Impact of gender on clinical presentation of chronic rhinosinusitis with and without polyposis [J].
Busaba, N. Y. ;
Sin, H-J ;
Salman, S. D. .
JOURNAL OF LARYNGOLOGY AND OTOLOGY, 2008, 122 (11) :1180-1184
[5]   Increased prevalence of interleukin-1 receptor antagonist gene polymorphism in patients with chronic rhinosinusitis [J].
Cheng, YK ;
Lin, CD ;
Chang, WC ;
Hwang, GY ;
Tsai, SW ;
Wan, L ;
Tsai, MH ;
Tsai, JJP ;
Tsai, FJ .
ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY, 2006, 132 (03) :285-290
[6]   SOME METHODS FOR STRENGTHENING THE COMMON X2 TESTS [J].
COCHRAN, WG .
BIOMETRICS, 1954, 10 (04) :417-451
[7]   Environmental risk factors and gender in nasal polyposis [J].
Collins, MM ;
Pang, YT ;
Loughran, S ;
Wilson, JA .
CLINICAL OTOLARYNGOLOGY, 2002, 27 (05) :314-317
[8]   Expression of leukotriene C4 synthase and plasminogen activator inhibitor 1 gene promoter polymorphisms in sinusitis [J].
de Alarcon, Alessandro ;
Steinke, John W. ;
Caughey, Robert ;
Barekzi, Elizabeth ;
Hise, Kathleen ;
Gross, Charles W. ;
Han, Joseph K. ;
Borish, Larry .
AMERICAN JOURNAL OF RHINOLOGY, 2006, 20 (05) :545-549
[9]   Genetic susceptibility to cancer - The role of polymorphisms in candidate genes [J].
Dong, Linda M. ;
Potter, John D. ;
White, Emily ;
Ulrich, Cornelia M. ;
Cardon, Lon R. ;
Peters, Ulrike .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2008, 299 (20) :2423-2436
[10]   Proinflammatory cytokine single nucleotide polymorphisms in nasal polyposis [J].
Erbek, Selim S. ;
Yurtcu, Erkan ;
Erbek, Seyra ;
Atac, F. Belgin ;
Sahin, Feride I. ;
Cakmak, Ozcan .
ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY, 2007, 133 (07) :705-709