Cell-free Protein Synthesis in a Microchamber Revealed the Presence of an Optimum Compartment Volume for High-order Reactions

被引：16

作者：

Okano, Taiji ^{[1
]}

Matsuura, Tomoaki ^{[1
,2
]}

Suzuki, Hiroaki ^{[1
,3
]}

Yomo, Tetsuya ^{[1
,4
,5
]}

机构：

[1] Japan Sci & Technol Agcy, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan

[3] Chuo Univ, Dept Precis Mech, Bunkyo Ku, Tokyo 1128551, Japan

[4] Osaka Univ, Grad Sch Informat Sci & Technol, Dept Bioinformat Engn, Suita, Osaka 5650871, Japan

[5] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

来源：

ACS SYNTHETIC BIOLOGY | 2014年 / 3卷 / 06期

关键词：

microchamber; compartmentalization; in vitro transcription and translation; PURE system; protein synthesis; BETA-GALACTOSIDASE; SELF-ORGANIZATION; KINETICS; ARRAY; SIZE; EXPRESSION;

D O I：

10.1021/sb400087e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of micro electromechanical systems (MEMS) to chemistry and biochemistry allows various reactions to be performed in microscale compartments. Here, we aimed to use the glass microchamber to study the compartment size dependency of the protein synthesis, one of the most important reactions in the cell. By encapsulating the cell-free protein synthesis system with different reaction orders in femtoliter microchambers, chamber size dependency of the reaction initiated with a constant copy number of DNA was investigated. We were able to observe the properties specific to the high order reactions in microcompartments with high precision and found the presence of an optimum compartment volume for a high-order reaction using real biological molecules.

引用

页码：347 / 352

页数：6

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