Identification of Lysine Succinylation Substrates and the Succinylation Regulatory Enzyme CobB in Escherichia coli

被引:218
作者
Colak, Gozde [1 ]
Xie, Zhongyu [1 ]
Zhu, Anita Y. [2 ]
Dai, Lunzhi [1 ]
Lu, Zhike [1 ]
Zhang, Yi [3 ]
Wan, Xuelian [3 ]
Chen, Yue [1 ]
Cha, Yoon H. [2 ]
Lin, Hening [2 ]
Zhao, Yingming [1 ,3 ]
Tan, Minjia [3 ]
机构
[1] Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA
[2] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA
[3] Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
基金
美国国家卫生研究院;
关键词
ACETYL-COA SYNTHETASE; SEQUENCE DATABASES; ROLES; PROKARYOTES; METABOLISM; ACTIVATION; COMPLEXES; PROTEINS;
D O I
10.1074/mcp.M113.031567
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Lysine succinylation is a newly identified protein posttranslational modification pathway present in both prokaryotic and eukaryotic cells. However, succinylation substrates and regulatory enzyme(s) remain largely unknown, hindering the biological study of this modification. Here we report the identification of 2,580 bacterial lysine succinylation sites in 670 proteins and 2,803 lysine acetylation (Kac) sites in 782 proteins, representing the first lysine succinylation dataset and the largest Kac dataset in wild-type E. coli. We quantified dynamic changes of the lysine succinylation and Kac substrates in response to high glucose. Our data showed that high-glucose conditions led to more lysine-succinylated proteins and enhanced the abundance of succinyllysine peptides more significantly than Kac peptides, suggesting that glucose has a more profound effect on succinylation than on acetylation. We further identified CobB, a known Sir2-like bacterial lysine deacetylase, as the first prokaryotic desuccinylation enzyme. The identification of bacterial CobB as a bifunctional enzyme with lysine desuccinylation and deacetylation activities suggests that the eukaryotic Kac-regulatory enzymes may have enzymatic activities on various lysine acylations with very different structures. In addition, it is highly likely that lysine succinylation could have unique and more profound regulatory roles in cellular metabolism relative to lysine acetylation under some physiological conditions. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
引用
收藏
页码:3509 / 3520
页数:12
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