Next-generation sequencing analysis for detecting human papillomavirus in oral verrucous carcinoma

被引：13

作者：

Samman, Manar ^{[1
]}

Wood, Henry ^{[1
]}

Conway, Caroline ^{[1
]}

Berri, Stefano ^{[1
]}

Pentenero, Monica ^{[2
]}

Gandolfo, Sergio ^{[2
]}

Cassenti, Adele ^{[3
]}

Cassoni, Paola ^{[3
]}

Al Ajlan, Abdulaziz ^{[4
]}

Barrett, A. William ^{[5
]}

Chengot, Preetha ^{[6
,7
]}

MacLennan, Kenneth ^{[1
]}

High, Alec S. ^{[6
,7
]}

Rabbitts, Pamela ^{[1
]}

机构：

[1] Univ Leeds, Leeds Inst Canc & Pathol, Leeds, W Yorkshire, England

[2] Univ Turin, Dept Oncol, Oral Med & Oral Oncol Unit, Turin, Italy

[3] Univ Turin, Dept Med Sci, Pathol Unit, Turin, Italy

[4] Natl Guard Hosp, Dept Pathol & Lab Med, Riyadh, Saudi Arabia

[5] Queen Victoria Hosp, Dept Histopathol, E Grinstead, W Sussex, England

[6] St James Univ Hosp, Dept Histopathol, Leeds LS2 9LU, W Yorkshire, England

[7] Univ Leeds, Leeds Dent Inst, Leeds, W Yorkshire, England

来源：

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY | 2014年 / 118卷 / 01期

关键词：

SQUAMOUS-CELL CARCINOMA; RISK-FACTOR; HEAD; NECK; DNA; PREVALENCE; INFECTION; CANCER; ASSOCIATION; SURVIVAL;

D O I：

10.1016/j.oooo.2014.03.018

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective. The etiology of oral verrucous carcinoma is unknown, and human papillomavirus 'involvement' remains contentious. The uncertainty can be attributed to varied detection procedures and difficulties in defining 'gold-standard' histologic criteria for diagnosing 'verrucous' lesions. Their paucity also hampers investigation. We aimed to analyze oral verrucous lesions for human papillomavirus (HPV) subtype genomes. Study Design. We used next-generation sequencing for the detection of papillomavirus sequences, identifying subtypes and computing viral loads. We identified a total of 78 oral verrucous cases (62 carcinomas and 16 hyperplasias). DNA was extracted from all and sequenced at a coverage between 2.5% and 13%. Results. An HPV-16 sequence was detected in 1 carcinoma and 1 hyperplasia, and an HPV-2 sequence was detected in 1 carcinoma out of the 78 cases, with viral loads of 2.24, 8.16, and 0.33 viral genomes per cell, respectively. Conclusions. Our results indicate no conclusive human papillomavirus involvement in oral verrucous carcinoma or hyperplasia.

引用

页码：117 / U213

页数：10

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