Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe

被引:11
作者
Fujita, Yasuko
Tohda, Hideki
Giga-Hama, Yuko
Takegawa, Kaoru [1 ]
机构
[1] Kagawa Univ, Fac Agr, Dept Life Sci, Miki, Kagawa 7610797, Japan
[2] Asahi Glass Co Ltd, Res Ctr, Yokohama, Kanagawa 221, Japan
关键词
fission yeast; inducible vector; heat shock protein; foreign gene expression;
D O I
10.1111/j.1567-1364.2006.00093.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A new, heat shock-inducible expression system based on an endogenous hsp16(+) promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16(+) promoter region was sufficient to drive expression of heterologous protein. The hsp16(+) promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16(+) promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe.
引用
收藏
页码:883 / 887
页数:5
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