A new sulfated β-galactan from clams with anti-HIV activity

被引：58

作者：

Amornrut, C

Toida, T

Imanari, T

Woo, ER

Park, H

Linhardt, R

Wu, SJ

Kim, YS

机构：

[1] Seoul Natl Univ, Inst Nat Prod Res, Seoul 110460, South Korea

[2] Chiba Univ, Fac Pharmaceut Sci, Chiba 2638522, Japan

[3] Chosun Univ, Coll Pharm, Kwangju 501759, South Korea

[4] Korea Inst Sci & Technol, Div Life Sci, Cheongryang 130650, South Korea

[5] Univ Iowa, Dept Chem & Biochem Engn, Iowa City, IA 52242 USA

[6] Univ Iowa, Dept Chem, Div Med & Nat Prod Chem, Iowa City, IA 52242 USA

来源：

CARBOHYDRATE RESEARCH | 1999年 / 321卷 / 1-2期

关键词：

clam polysaccharide; beta-(1 -> 3)-linked galactan sulfate; anti-HIV activity;

D O I：

10.1016/S0008-6215(99)00188-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new polysaccharide composed of galactan sulfate with a beta-(l --> 3)-glycosidic linkage has been isolated from the marine clam species Meretrix petechialis. The polysaccharide was homogeneous in its composition containing D-galactose. The glycosidic linkage was examined by 2D DQF-COSY and 2D NOESY spectroscopy. The coupling constant of anomeric proton was 7.8 Hz, suggesting a beta-galacto configuration. The downfield shift of H-2 of galactose residue demonstrated the presence of 2-O-sulfonate group. TQF-COSY confirmed that the C-6 position was substituted with a sulfonate group. The anti-HIV activity of the polysaccharides has been evaluated by the inhibition of syncytia formation. The fusion index and percentage fusion inhibition of sulfated galactan were 0.34 and 56% at 200 mu g/mL. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：121 / 127

页数：7

共 21 条

[1] A COMPREHENSIVE PROCEDURE FOR PREPARATION OF PARTIALLY METHYLATED ALDITOL ACETATES FROM GLYCOPROTEIN CARBOHYDRATES [J].