The construction of glucose biosensor based on crystalline iridium(III)-containing coordination polymers with fiber-optic detection

被引：32

作者：

Ho, Mei-Lin ^{[1
]}

Wang, Jing-Chang ^{[1
]}

Wang, Ting-Yi ^{[1
]}

Lin, Chun-Yen ^{[1
]}

Zhu, Jian Fan ^{[1
]}

Chen, Yi-An ^{[1
]}

Chen, Tsai-Chen ^{[1
]}

机构：

[1] Soochow Univ, Dept Chem, Taipei 11102, Taiwan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 190卷

关键词：

Coordination polymers; Biosensor; Glucose; Serum; Fiber-optics; Layer-by-layer method; REAGENTLESS OPTICAL BIOSENSOR; OXYGEN-SENSING PROPERTIES; OXIDASE; MEMBRANE; NANOPARTICLES; COMPLEXES; PLATFORM; SERUM;

D O I：

10.1016/j.snb.2013.08.100

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This study reports a simple and sensitive method for phosphorescence detection of glucose concentration in beverage samples and human serum. This optical sensor was constructed based on sensing substrate made of crystalline iridium(III)-containing coordination polymers doped in sol-gel matrix and built up under glucose oxidase encapsulated in hydrogel and then immobilized on egg membrane, using the layer-by-layer method. The detection mechanism is based on the depletion of oxygen content upon exposure of glucose solution with a concomitant increase in phosphorescence of coordination polymers. The effects of buffer concentration, pH, interference and temperature have been studied in detail. Under optimum conditions, the luminescence changing rate revealed a good linear relationship from 0.05 mM to 5.0 mM (y = 1.058 [glucose] + 0.6591, R-2 = 0.9935) with a detection limit of 0.01 mM. This method has also been successfully applied to the determination of glucose in real samples. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：479 / 485

页数：7

共 28 条

[1] Encapsulation of glucose oxidase and an oxygen-quenched fluorophore in polyelectrolyte-coated calcium alginate microspheres as optical glucose sensor systems [J].