Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development

被引：33

作者：

Ishiuchi, Takashi ^{[1
]}

Ohishi, Hiroaki ^{[1
]}

Sato, Tetsuya ^{[2
]}

Kamimura, Satoshi ^{[3
,5
]}

Yorino, Masayoshi ^{[4
]}

Abe, Shusaku ^{[1
]}

Suzuki, Atsushi ^{[4
]}

Wakayama, Teruhiko ^{[3
]}

Suyama, Mikita ^{[2
]}

Sasaki, Hiroyuki ^{[1
]}

机构：

[1] Kyushu Univ, Med Inst Bioregulat, Div Epigen & Dev, Fukuoka, Fukuoka 8128582, Japan

[2] Kyushu Univ, Med Inst Bioregulat, Div Bioinformat, Fukuoka, Fukuoka 8128582, Japan

[3] Univ Yamanashi, Adv Biotechnol Ctr, Kofu, Yamanashi 4008510, Japan

[4] Kyushu Univ, Med Inst Bioregulat, Div Organogenesis & Regenerat, Fukuoka, Fukuoka 8128582, Japan

[5] Natl Inst Radiol Sci, Dept Basic Med Sci Radiat Damages, Chiba 2638555, Japan

来源：

CELL REPORTS | 2019年 / 27卷 / 06期

关键词：

SELF-RENEWAL; MOUSE; DIFFERENTIATION; DERIVATION; EXPRESSION; EMBRYOS; MICE; PROLIFERATION; PLURIPOTENCY; MAINTENANCE;

D O I：

10.1016/j.celrep.2019.04.028

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Placental development is a key event in mammalian reproduction and embryogenesis. However, the molecular basis underlying placental development is not fully understood. Here, we conduct a forward genetic screen to identify regulators for extraembryonic development and identify Zfp281 as a key factor. Zfp281 overexpression in mouse embryonic stem cells facilitates the induction of trophoblast stem-like cells. Zfp281 is preferentially expressed in the undifferentiated trophoblast stem cell population in an FGF-dependent manner, and disruption of Zfp281 in mice causes severe defects in early placental development. Consistently, Zfp281-depleted trophoblast stem cells exhibit defects in maintaining the transcriptome and differentiation capacity. Mechanistically, Zfp281 interacts with MLL or COMPASS subunits and occupies the promoters of its target genes. Importantly, ZNF281, the human ortholog of this factor, is required to stabilize the undifferentiated status of human trophoblast stem cells. Thus, we identify Zfp281 as a conserved factor for the maintenance of trophoblast stem cell plasticity.

引用

页码：1742 / +

页数：19

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