Methylene blue reduces pulmonary oedema and cyclo-oxygenase products in endotoxaemic sheep

被引:17
作者
Evgenov, OV
Evgenov, NV
Mollnes, TE
Bjertnaes, LJ
机构
[1] Univ Tromso, Fac Med, Dept Anesthesiol, Tromso, Norway
[2] Univ Tromso, Fac Med, Dept Internal Med, Tromso, Norway
[3] Nordland Cent Hosp, Dept Immunol & Transfus Med, Bodo, Norway
关键词
endotoxin; extravascular lung water; methylene blue; pulmonary circulation; 6-keto-prostaglandin F-1 alpha; thromboxane B-2;
D O I
10.1183/09031936.02.00932001
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
The authors recently demonstrated that methylene blue (MB), an inhibitor of the nitric oxide (NO) pathway, reduces the increments in pulmonary capillary pressure, lung lymph flow and protein clearance in endotoxaemic sheep. In the present study, the authors examined whether MB influences pulmonary haemodynamics and accumulation of extravascular lung water (EVLW) by mechanisms other than the NO pathway. Sixteen awake, chronically-instrumented sheep randomly received either an intravenous injection of MB 10 mg-kg(-1) or isotonic saline. Thirty minutes later, all sheep received an intravenous infusion of Escherichia coli endotoxin 1 Ag (.) kg(-1) for 20 min and either an intravenous infusion of MB 2.5 mg-kg(-1)-h(-1) or isotonic saline for 6 h. MB markedly attenuated the endotoxin-induced pulmonary hypertension and right ventricular failure, and reduced the accumulation of EVLW. Moreover, MB reduced the increments in plasma thromboxane B-2 and 6-keto-prostaglandin F-1alpha, and abolished the febrile response. However, MB had no effect on the changes in circulating neutrophils, serum hyaluronan, and total haemolytic activity of the alternative complement pathway. The authors conclude that in sheep, methylene blue attenuates the endotoxin-induced pulmonary hypertension and oedema, at least in part, by inhibiting the cyclo-oxygenase products of arachidonic acid. This is a novel effect of methylene blue in vivo.
引用
收藏
页码:957 / 964
页数:8
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