Activity-Based Probe for Screening of High-Colibactin Producers from Clinical Samples

被引：17

作者：

Hirayama, Yuichiro ^{[1
]}

Tsunematsu, Yuta ^{[1
]}

Yoshikawa, Yuko ^{[2
]}

Tamafune, Ryota ^{[1
]}

Matsuzaki, Nobuo ^{[1
]}

Iwashita, Yuji ^{[3
]}

Ohnishi, Ippei ^{[4
]}

Tanioka, Fumihiko ^{[4
]}

Sato, Michio ^{[1
]}

Miyoshi, Noriyuki ^{[5
]}

Mutoh, Michihiro ^{[6
]}

Ishikawa, Hideki ^{[7
]}

Sugimura, Haruhiko ^{[3
]}

Wakabayashi, Keiji ^{[8
]}

Watanabe, Kenji ^{[1
]}

机构：

[1] Univ Shizuoka, Dept Pharmaceut Sci, Shizuoka 4228526, Japan

[2] Nippon Vet & Life Sci Univ, Fac Vet Sci, Sch Vet Med, Tokyo 1808602, Japan

[3] Hamamatsu Univ Sch Med, Dept Tumor Pathol, Shizuoka 4313192, Japan

[4] Iwata City Hosp, Div Pathol, Iwata 4388550, Japan

[5] Univ Shizuoka, Grad Sch Nutr & Environm Sci, Shizuoka 4228526, Japan

[6] Natl Canc Ctr, Ctr Publ Hlth Sci, Epidemiol & Prevent Div, Tokyo 1040045, Japan

[7] Kyoto Prefectural Univ Med, Dept Mol Targeting Canc Prevent, Kyoto 6028566, Japan

[8] Univ Shizuoka, Grad Div Nutr & Environm Sci, Shizuoka 4228526, Japan

来源：

ORGANIC LETTERS | 2019年 / 21卷 / 12期

关键词：

PRODRUG ACTIVATION MECHANISM; GENE-CLUSTER; IN-VIVO; BIOSYNTHESIS; METABOLOMICS;

D O I：

10.1021/acs.orglett.9b01345

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

While high-colibactin-producing Escherichia coil is thought to be associated with colorectal oncogenesis, this study is complicated part due to an inability to isolate colibactin adequately. Here, we created fluorescent probes activated by ClbP, the colibactin-maturing peptidase, to identify high-colibactin-producing strains. Our probe served as a valuable clinical diagnostic tool that allowed simple high throughput diagnostic screening of clinical samples. Furthermore, the probe also allowed identification of high-colibactin producers that would help advance our understanding of colibactin biosynthesis.

引用

页码：4490 / 4494

页数：5

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