Identification and Biological Characterization of Leishmania (Viannia) guyanensis Isolated from a Patient with Tegumentary Leishmaniasis in Goias, a Nonendemic Area for This Species in Brazil

被引:5
作者
Pires, Alause da Silva [1 ]
Borges, Arissa Felipe [1 ]
Coelho, Adriano Cappellazzo [2 ]
Dorta, Miriam Leandro [1 ]
Lino Junior, Ruy de Souza [1 ]
de Araujo Pereira, Ledice Inacia [1 ]
Pinto, Sebastiao Alves [3 ,4 ]
Pelli de Oliveira, Milton Adriano [1 ]
de Matos, Grazzielle Guimaraes [1 ]
Abrahamsohn, Ises A. [5 ]
Uliana, Silvia Reni B. [2 ]
Collet de Araujo Lima, Gloria Maria [5 ]
Ribeiro-Dias, Fatima [1 ]
机构
[1] Univ Fed Goias, Inst Patol Trop & Saude Publ, BR-74605050 Goiania, Go, Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, BR-05508 Sao Paulo, SP, Brazil
[3] Univ Fed Goias, Inst Goiano Oncol & Hematol, BR-74605050 Goiania, Go, Brazil
[4] Univ Fed Goias, Fac Med, BR-74605050 Goiania, Go, Brazil
[5] Univ Sao Paulo, BR-05508 Sao Paulo, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
AMERICAN CUTANEOUS LEISHMANIASIS; GEOGRAPHICAL-DISTRIBUTION; MATO-GROSSO; WORLD; PSYCHODIDAE; INFECTION; DIPTERA; STATE; PHLEBOTOMINAE; EPIDEMIOLOGY;
D O I
10.1155/2015/350764
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The aim of this study was to characterize clinical field isolates of Leishmania spp. obtained from patients with American Tegumentary Leishmaniasis (ATL) who live in Goias state, Brazil. The presumed areas of infection were in Goias, Tocantins, and Para states. Three isolates of parasites were identified as L. (Viannia) braziliensis and one as L. (V.) guyanensis. The in vitro growth profiles were found to be similar for all parasites. Nevertheless, in C57BL/6 mice, L. (V.) guyanensis infection was better controlled than L. (V.) braziliensis. Yet in C57BL/6 mice deficient in interferon gamma, L. (V.) guyanensis lesions developed faster than those caused by L. (V.) braziliensis isolates. In BALB/c mice, the development of lesions was similar for isolates from both species; however, on the 11th week of infection, amastigotes could not be observed in macrophages from L. (V.) guyanensis-infected mice. Thus, L. (V.) guyanensis can be circulating in Goias, a state where autochthonous cases of this species had not yet been reported. Considering the difficulties to differentiate L. (V.) guyanensis from L. (V.) braziliensis at the molecular, morphological, and clinical (human and murine models) levels, the presence of L. (V.) guyanensis infections is possibly underestimated in several regions of Brazil.
引用
收藏
页数:11
相关论文
共 45 条
[1]   Leishmaniasis Worldwide and Global Estimates of Its Incidence [J].
Alvar, Jorge ;
Velez, Ivan D. ;
Bern, Caryn ;
Herrero, Merce ;
Desjeux, Philippe ;
Cano, Jorge ;
Jannin, Jean ;
den Boer, Margriet .
PLOS ONE, 2012, 7 (05)
[2]  
[Anonymous], 2007, MAN VIG LEISHM TEG A
[3]  
[Anonymous], 2010, MAN VIG LEISHM TEG A
[4]   Influence of Leishmania (Viannia) species on the response to antimonial treatment in patients with American tegumentary leishmaniasis [J].
Arevalo, Jorge ;
Ramirez, Luis ;
Adaui, Vanessa ;
Zimic, Mirko ;
Tulliano, Gianfranco ;
Miranda-Verastegui, Cesar ;
Lazo, Marcela ;
Loayza-Muro, Raul ;
De Doncker, Simonne ;
Maurer, Anne ;
Chappuis, Francois ;
Dujardin, Jean-Claude ;
Llanos-Cuentas, Alejandro .
JOURNAL OF INFECTIOUS DISEASES, 2007, 195 (12) :1846-1851
[5]   The leishmaniases as emerging and reemerging zoonoses [J].
Ashford, RW .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2000, 30 (12-13) :1269-1281
[6]   Ecology of sand flies (Diptera:Psychodidae:Phlebotominae) in the north of the State of Mato Grosso, Brazil [J].
Azevedo, ACR ;
Souza, NA ;
Meneses, CRV ;
Costa, WA ;
Costa, SM ;
Lima, JB ;
Rangel, EF .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2002, 97 (04) :459-464
[7]   New Insights on Taxonomy, Phylogeny and Population Genetics of Leishmania (Viannia) Parasites Based on Multilocus Sequence Analysis [J].
Boite, Mariana C. ;
Mauricio, Isabel L. ;
Miles, Michael A. ;
Cupolillo, Elisa .
PLOS NEGLECTED TROPICAL DISEASES, 2012, 6 (11)
[8]   New PCR assay using glucose-6-phosphate dehydrogenase for identification of Leishmania species [J].
Castilho, TM ;
Shaw, JJ ;
Floeter-Winter, LM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (02) :540-546
[9]   Disseminated cutaneous leishmaniasis due to Leishmania guyanensis:: Case of a patient with 425 lesions [J].
Couppié, P ;
Clyti, E ;
Sainte-Marie, D ;
Dedet, JP ;
Carme, B ;
Pradinaud, R .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2004, 71 (05) :558-560
[10]   Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil [J].
Cupolillo, E ;
Brahim, LR ;
Toaldo, CB ;
de Oliveira-Neto, MP ;
de Brito, MEF ;
Falqueto, A ;
Naiff, MD ;
Grimaldi, G .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :3126-3132