Whole genome shotgun sequence of Bacillus paralicheniformis strain KMS 80, a rhizobacterial endophyte isolated from rice (Oryza sativa L.)

被引:26
作者
Annapurna, Kannepalli [1 ]
Govindasamy, Venkadasamy [1 ]
Sharma, Meenakshi [1 ]
Ghosh, Arpita [2 ]
Chikara, Surendra K. [2 ]
机构
[1] Indian Agr Res Inst, ICAR, Div Microbiol, New Delhi 110012, India
[2] Eurofins Genom India Private Ltd, Bangalore 560048, Karnataka, India
关键词
Rice (Oryza sativa L.); Endophyte; Diazotroph; Bacillus paralicheniformis; Illumina; NGS; Whole genome sequencing;
D O I
10.1007/s13205-018-1242-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacillus paralicheniformis strain KMS 80 (MTCC No. 12704) is an isolate from the root tissues of rice (Oryza sativa L.) that displays biological nitrogen fixation and plant growth promoting abilities. Here, we report the complete genome sequence of this strain, which contains 4,566,040 bp, 4424 protein-coding genes, 8692 promoter sequences, 67 tRNAs, 20 rRNA genes with six copies of 5S rRNAs along with a single copy of 16S-23S rRNA and genome average GC-content of 45.50%. Twenty one genes involved in nitrogen metabolism pathway and two main transcriptional factor genes, glnR and tnrA responsible for regulation of nitrogen fixation in Bacillus sp. were predicted from the whole genome of strain KMS 80. Analysis of the similar to 4.57 Mb genome sequence will give support to understand the genetic determinants of host range, endophytic colonization behaviour as well as to enhance endophytic nitrogen fixation and other plant beneficial role of B. paralicheniformis in rice.
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