Detection and interpretation of redox potential optima in the catalytic activity of enzymes

被引：43
作者：
Elliott, SJ
Léger, C
Pershad, HR
Hirst, J
Heffron, K
Ginet, N
Blasco, F
Rothery, RA
Weiner, JH
Armstrong, FA
机构：
[1] Univ Oxford, Inorgan Chem Lab, Oxford OX1 3QR, England
[2] MRC, Dunn Human Nutr Unit, Cambridge CB2 2XY, England
[3] CNRS, Chim Bacterienne Lab, IBSM, F-13402 Marseille, France
[4] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2002年 / 1555卷 / 1-3期
基金：
英国生物技术与生命科学研究理事会;
关键词：
oxidoreductase; voltammetry; respiration; electron transport; redox enzyme; electrochemistry;
D O I：
10.1016/S0005-2728(02)00254-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It is no surprise that the catalytic activity of electron-transport enzymes may be optimised at certain electrochemical potentials in ways that are analogous to observations of pH-rate optima. This property is observed clearly in experiments in which an enzyme is adsorbed on an electrode surface which can supply or receive electrons rapidly and in a highly controlled manner. In such a way, the rate of catalysis can be measured accurately as a function of the potential (driving force) that is applied. In this paper, we draw attention to a few examples in which this property has been observed in enzymes that are associated with membrane-bound respiratory chains, and we discuss its possible origins and implications for in vivo regulation. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：54 / 59
页数：6
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