Improved cellular immune response elicited by a ubiquitin-fused ESAT-6 DNA vaccine against Mycobacterium tuberculosis

被引：15

作者：

Wang, Qing-min ^{[1
]}

Kang, Lin ^{[2
]}

Wang, Xiao-Hua ^{[1
]}

机构：

[1] Inst Navy Med Res, Ship Environm Hlth Div, Shanghai 200433, Peoples R China

[2] Hebei Med Univ, Dept Anat, Shijiazhuang 050011, Hebei Province, Peoples R China

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2009年 / 53卷 / 07期

关键词：

immune response; Mycobacterium tuberculosis; UbGR-ESAT-6 fusion DNA vaccine; MHC CLASS-I; PROTECTIVE EFFICACY; T-CELL; INDUCTION; IMMUNOGENICITY; PROTEIN; IMMUNIZATION; TH1; ENHANCEMENT; EXPRESSION;

D O I：

10.1111/j.1348-0421.2009.00138.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study evaluated the immune response elicited by a ubiquitin-fused ESAT-6 DNA vaccine against Mycobacterium tuberculosis. BALB/c mice were vaccinated with plasmid DNA encoding ESAT-6 protein, ubiquitin-fused ESAT-6 DNA vaccine (UbGR-ESAT-6), pcDNA3-ubiquitin and blank vector, respectively. ESAT-6 DNA vaccine immunization induced a Thl-polarized immune response. The production of Thl-type cytokine (IFN-gamma) and proliferative T-cell responses was enhanced significantly in mice immunized with UbGR-ESAT-6 fusion DNA vaccine, compared to non-fusion DNA vaccine. This fusion DNA vaccine also resulted in an increased relative ratio of IgG(2a) to IgG(l) and the cytotoxicity of T cells. Thus, the present study demonstrated that the UbGR-ESAT-6 fusion DNA vaccine inoculation improved antigen-specific cellular immune responses, which is helpful for protection against tuberculosis infection.

引用

页码：384 / 390

页数：7

共 31 条

[1] Induction of a type 1 immune response to a recombinant antigen from Mycobacterium tuberculosis expressed in Mycobacterium vaccae [J].

AbouZeid, C ;

Gares, MP ;

Inwald, J ;

Janssen, R ;

Zhang, Y ;

Young, DB ;

Hetzel, C ;

Lamb, JR ;

Baldwin, SL ;

Orme, IM ;

Yeremeev, V ;

Nikonenko, BV ;

Apt, AS .

INFECTION AND IMMUNITY, 1997, 65 (05) :1856-1862

[2] The success and failure of BCG - implications for a novel tuberculosis vaccine [J].

Andersen, P ;

Doherty, TM .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (08) :656-662

[3] Therapeutic vaccination of rabbits with a ubiquitin-fused papillomavirus E1, E2, E6 and E7 DNA vaccine [J].

Brandsma, Janet L. ;

Shlyankevich, Mark ;

Zelterman, Daniel ;

Su, Yuhua .

VACCINE, 2007, 25 (33) :6158-6163

[4] ESAT-6 subunit vaccination against Mycobacterium tuberculosis [J].

Brandt, L ;

Elhay, M ;

Rosenkrands, I ;

Lindblad, EB ;

Andersen, P .

INFECTION AND IMMUNITY, 2000, 68 (02) :791-795

[5]

Cardona PJ, 1999, SCAND J IMMUNOL, V49, P362

[6] A tuberculosis vaccine based on phosphoantigens and fusion proteins induces distinct γδ and αβ T cell responses in primates [J].

Cendron, Delphine ;

Ingoure, Sophie ;

Martino, Angelo ;

Casetti, Rita ;

Horand, Francoise ;

Romagne, Francois ;

Sicard, Heleme ;

Fournie, Jean-Jacques ;

Poccia, Fabrizio .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2007, 37 (02) :549-565

[7]

Chow YH, 1998, J IMMUNOL, V160, P1320

[8]

COTTEN M, 1994, GENE THER, V1, P239

[9]

DANKO I, 1994, GENE THER, V1, P114

[10] Targeting antigen to MHC class I and class II antigen presentation pathways for malaria DNA vaccines [J].

Dobano, Carlota ;

Rogers, William O. ;

Gowda, Kalpana ;

Doolan, Denise L. .

IMMUNOLOGY LETTERS, 2007, 111 (02) :92-102

← 1 2 3 4 →