Bench-to-bedside optimization of mesenchymal stem cell isolation, processing, and expansion for in vivo administration

被引：3

作者：

Antebi, Ben ^{[1
]}

Walker, Kerfoot P., III ^{[1
,2
]}

Mohammadipoor, Arezoo ^{[1
,2
]}

Rodriguez, Luis A., II ^{[1
]}

Moore, Robbie K. ^{[1
]}

Cancio, Leopoldo C. ^{[1
]}

Batchinsky, Andriy I. ^{[1
,3
]}

机构：

[1] US Army Inst Surg Res, San Antonio, TX 78234 USA

[2] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA

[3] Geneva Fdn, Tacoma, WA 98402 USA

来源：

REGENERATIVE MEDICINE | 2019年 / 14卷 / 04期

关键词：

bone marrow; bone marrow aspirate; colony-forming unit fibroblasts; fetal bovine serum; mesenchymal stem cells; platelet lysate; population doublings; seeding density; FETAL BOVINE SERUM; HUMAN PLATELET LYSATE; BONE-MARROW; STROMAL CELLS; TELOMERE LENGTH; CULTURE; BLOOD; DIFFERENTIATION; NUMBER;

D O I：

10.2217/rme-2018-0043

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Aim: In this study, we aimed at identifying the optimal conditions for isolation, processing and expansion of mesenchymal stem cells (MSCs). Methods: Porcine bone marrow was obtained from either small-or large-volume bone marrow aspirate (BMA). Next, three BMA processing methods were compared. Finally, the best condition was selected from various culture parameters, including basal media, supplementation and seeding density. Results: Our results demonstrate that a small-volume BMA and direct plating yields significantly higher concentration of MSCs. Basal media supplementation with 10% platelet lysate and seeding density of 1000 cells/cm(2) can generate large numbers of multipotent MSCs with augmented function and low population doublings. Conclusion: This work provides guidance for preparation of robust MSCs for future clinical trials.

引用

页码：279 / 293

页数：15

共 48 条

[1] The promise of mesenchymal stem cell therapy for acute respiratory distress syndrome [J].