Bench-to-bedside optimization of mesenchymal stem cell isolation, processing, and expansion for in vivo administration

被引：3
作者：
Antebi, Ben [1 ]
Walker, Kerfoot P., III [1 ,2 ]
Mohammadipoor, Arezoo [1 ,2 ]
Rodriguez, Luis A., II [1 ]
Moore, Robbie K. [1 ]
Cancio, Leopoldo C. [1 ]
Batchinsky, Andriy I. [1 ,3 ]
机构：
[1] US Army Inst Surg Res, San Antonio, TX 78234 USA
[2] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA
[3] Geneva Fdn, Tacoma, WA 98402 USA
来源：
REGENERATIVE MEDICINE | 2019年 / 14卷 / 04期
关键词：
bone marrow; bone marrow aspirate; colony-forming unit fibroblasts; fetal bovine serum; mesenchymal stem cells; platelet lysate; population doublings; seeding density; FETAL BOVINE SERUM; HUMAN PLATELET LYSATE; BONE-MARROW; STROMAL CELLS; TELOMERE LENGTH; CULTURE; BLOOD; DIFFERENTIATION; NUMBER;
D O I：
10.2217/rme-2018-0043
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: In this study, we aimed at identifying the optimal conditions for isolation, processing and expansion of mesenchymal stem cells (MSCs). Methods: Porcine bone marrow was obtained from either small-or large-volume bone marrow aspirate (BMA). Next, three BMA processing methods were compared. Finally, the best condition was selected from various culture parameters, including basal media, supplementation and seeding density. Results: Our results demonstrate that a small-volume BMA and direct plating yields significantly higher concentration of MSCs. Basal media supplementation with 10% platelet lysate and seeding density of 1000 cells/cm(2) can generate large numbers of multipotent MSCs with augmented function and low population doublings. Conclusion: This work provides guidance for preparation of robust MSCs for future clinical trials.
引用
收藏
页码：279 / 293
页数：15
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