Irreversible lysozyme inactivation and aggregation induced by stirring:: kinetic study and aggregates characterisation

被引：19
作者：
Colombié, S
Gaunand, A
Rinaudo, M
Lindet, B
机构：
[1] Ecole Natl Super Mines, CEREP, F-75006 Paris, France
[2] Ctr Rech Macromol Vegetales, F-38041 Grenoble 9, France
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 04期
关键词：
enzyme inactivation; lysozyme; lysozyme aggregation; stirring;
D O I：
10.1023/A:1005655005780
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Stirring strongly enhanced irreversible inactivation and aggregation of lysozyme being studied as a model enzyme. From 0 to 740 rpm (equivalent to impeller tip speeds from 0 to 0.77 m s(-1)), the inactivation kinetic constant was proportional to the power imparted by the impeller. Collisions between inactive and native molecules induced inactivation of the latter and led to lysozyme aggregation. These fractal aggregates of lysozyme were made of monomers, dimers and trimers.
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收藏
页码：277 / 283
页数：7
相关论文
共 15 条
[1]   THE MECHANISM OF IRREVERSIBLE ENZYME INACTIVATION AT 100-DEGREES-C [J].
AHERN, TJ ;
KLIBANOV, AM .
SCIENCE, 1985, 228 (4705) :1280-1284
[2]  
AHERN TJ, 1988, METHOD BIOCHEM ANAL, V33, P91
[3]   Lysozyme inactivation by inert gas bubbling:: kinetics in a bubble column reactor [J].
Caussette, M ;
Gaunand, A ;
Planche, H ;
Colombié, S ;
Monsan, P ;
Lindet, B .
ENZYME AND MICROBIAL TECHNOLOGY, 1999, 24 (07) :412-418
[4]   Pectinmethylesterase inactivation by bubbling with N2 [J].
Caussette, M ;
Gaunand, A ;
Planche, H ;
Colombie, S ;
Monsan, P ;
Lindet, B .
BIOTECHNOLOGY TECHNIQUES, 1998, 12 (07) :561-564
[5]   The self catalytic enzyme inactivation induced by solvent stirring: a new example of protein conformational change induction [J].
Caussette, M ;
Planche, H ;
Delepine, S ;
Monsan, P ;
Gaunand, A ;
Lindet, B .
PROTEIN ENGINEERING, 1997, 10 (10) :1235-1240
[6]   THERMAL STABILITIES OF GLOBULAR-PROTEINS [J].
DILL, KA ;
ALONSO, DOV ;
HUTCHINSON, K .
BIOCHEMISTRY, 1989, 28 (13) :5439-5449
[7]   INACTIVATION OF ENZYMES BY ORGANIC-SOLVENTS - NEW TECHNIQUE WITH WELL-DEFINED INTERFACIAL AREA [J].
GHATORAE, AS ;
BELL, G ;
HALLING, PJ .
BIOTECHNOLOGY AND BIOENGINEERING, 1994, 43 (04) :331-336
[8]  
JAENICKE R, 1967, J POLYM SCI POL SYM, P2143
[9]   EFFECT OF SUBUNIT DISSOCIATION, DENATURATION, AGGREGATION, COAGULATION, AND DECOMPOSITION ON ENZYME INACTIVATION KINETICS .1. 1ST-ORDER BEHAVIOR [J].
LENCKI, RW ;
ARUL, J ;
NEUFELD, RJ .
BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (11) :1421-1426
[10]   EFFECT OF SUBUNIT DISSOCIATION, DENATURATION, AGGREGATION, COAGULATION, AND DECOMPOSITION ON ENZYME INACTIVATION KINETICS .2. BIPHASIC AND GRACE PERIOD BEHAVIOR [J].
LENCKI, RW ;
ARUL, J ;
NEUFELD, RJ .
BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (11) :1427-1434
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