The central clock controls the daily rhythm of Aqp5 expression in salivary glands

被引：6

作者：

Uchida, Hitoshi ^{[1
,2
]}

Nakamura, Takahiro J. ^{[3
,4
]}

Takasu, Nana N. ^{[1
]}

Obana-Koshino, Aya ^{[2
]}

Ono, Hitomi ^{[2
]}

Todo, Takeshi ^{[5
]}

Sakai, Takayoshi ^{[2
]}

Nakamura, Wataru ^{[1
]}

机构：

[1] Nagasaki Univ, Dept Oral Chrono Physiol, Grad Sch Biomed Sci, 1-7-1 Sakamoto, Nagasaki, Nagasaki 8528588, Japan

[2] Osaka Univ, Grad Sch Dent, Dept Oral Facial Disorders, Suita, Osaka 5650871, Japan

[3] Meiji Univ, Sch Agr, Dept Life Sci, Kawasaki, Kanagawa 2148571, Japan

[4] Teikyo Heisei Univ, Fac Pharmaceut Sci, Tokyo 1648530, Japan

[5] Osaka Univ, Grad Sch Med, Dept Radiat Biol & Med Genet, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF PHYSIOLOGICAL SCIENCES | 2018年 / 68卷 / 04期

关键词：

Circadian rhythm; Salivary gland; Suprachiasmatic nucleus; Aquaporin; 5; Period; 2; PERIPHERAL CIRCADIAN OSCILLATORS; AQUAPORIN WATER CHANNELS; SUPRACHIASMATIC NUCLEUS; SUBMANDIBULAR-GLANDS; NERVE-STIMULATION; ACINAR-CELLS; NULL MICE; MOUSE; SECRETION; RATS;

D O I：

10.1007/s12576-017-0540-1

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Salivary secretion displays day-night variations that are controlled by the circadian clock. The central clock in the suprachiasmatic nucleus (SCN) regulates daily physiological rhythms by prompting peripheral oscillators to adjust to changing environments. Aquaporin 5 (Aqp5) is known to play a key role in salivary secretion, but the association between Aqp5 and the circadian rhythm is poorly understood. The aim of our study was to evaluate whether Aqp5 expression in submandibular glands (SMGs) is driven by the central clock in the SCN or by autonomous oscillations. We observed circadian oscillations in the activity of period circadian protein homolog 2 and luciferase fusion protein (PER2::LUC) in cultured SMGs with periodicity depending on core clock genes. A daily rhythm was detected in the expression profiles of Aqp5 in SMGs in vivo. In cultured SMGs ex vivo, clock genes showed distinct circadian rhythms, whereas Aqp5 expression did not. These data indicate that daily Aqp5 expression in the mouse SMG is driven by the central clock in the SCN.

引用

页码：377 / 385

页数：9

共 45 条

[1] EFFECTS OF ELECTRICAL AND PHARMACOLOGICAL STIMULATION ON TYPES OF PROTEINS SECRETED BY RAT PAROTID AND SUB-MANDIBULAR GLANDS [J].