Reduced MTA1 expression by RNAi inhibits in vitro invasion and migration of esophageal squamous cell carcinoma cell line

被引:46
作者
Qian, Haili
Lu, Ning
Xue, Liyan
Liang, Xiao
Zhang, Xueyan
Fu, Ming
Xie, Yongqiang
Zhan, Qimin
Liu, Zhihua
Lin, Chen
机构
[1] Chinese Acad Med Sci, Inst Canc, Canc Hosp, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
[2] Peking Union Med Coll, Beijing 100021, Peoples R China
[3] Chinese Acad Med Sci, Inst Canc, Canc Hosp, Dept Pathol, Beijing 100021, Peoples R China
关键词
metastasis-associated gene 1 (Mta1); esophageal squamous cell carcinoma; invasion; metastasis; tissue array; immunohistochemistry; microarray;
D O I
10.1007/s10585-006-9005-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To distinguish aggressive esophageal squamous cell carcinoma from indolent disease is the important clinical challenge. Studies have indicated that metastasis-associated gene 1(Mta1) played a role in the process of metastasis of carcinoma. The overexpression of Mta1 gene has been found in a variety of tumors. To identify the detailed roles of MTA1 protein in the carcinogenesis of esophageal squamous cell carcinoma, this study analyzed the pathological specimens on tissue microarray derived from 72 patients using immunohistochemistry. MTA1 expression increased in the nuclear with the development of esophageal squamous cell carcinoma from normal epithelial cell, dysplasia, to invasive cancer. In biological studies with human esophageal squamous cell carcinoma cell line, MTA1 plays its roles to promote cancer cell invasion, adhesion and movement. RNA interference (RNAi) against MTA1 decreased the malignant phenotypes. Gene microarray analysis revealed some metastasis-associated genes were altered by MTA1 RNAi. This study started an effective beginning to explore metastasis mechanisms and cancer gene therapy strategy targeting MTA1.
引用
收藏
页码:653 / 662
页数:10
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