Optimising the determination of maize endosperm vitreousness by a rapid non-destructive image analysis technique

A rapid non-destructive image analysis technique was developed and optimised for the determination of maize endosperm vitreousness without the need for sample preparation. Maize kernel translucency measurements were optimised for a light system consisting of positioning whole kernels on top of round illuminated areas smaller than the projected areas of the kernels, allowing light to shine through the kernels. A correction factor to allow for constant illumination of kernels was developed to adjust for kernel size variation in relation to constant light area. The intensity of translucency in maize is linearly correlated to the percentage of kernel illumination (r = 0.99, p < 0.001). Significant correlations were found between corrected translucency values and endosperm yields determined by hand dissection. These were: translucency as a percentage of the whole kernel and vitreous endosperm (mass%), r = 0.77; translucency as a percentage of the whole kernel and opaque endosperm (mass%), (r = -0.72); translucency as a percentage of endosperm and vitreous endosperm (mass%), r = 0.81; and translucency as a percentage of endosperm and opaque endosperm (mass%), r = -0.77; with n = 245 in all instances. Translucency varied by 29.5% between the lowest and highest values, and vitreous endosperm (mass%) varied by 16.8% between the lowest and highest values. Correlations increased significantly after corrections for kernel thickness. A thickness increase of 1 mm in the maize will cause a decrease of 21.86% in translucency and vice versa. The method allows for large samples (at least 49 kernels min(-1)) to be analysed for individual vitreous and opaque endosperm contents with no interferences with kernel structure. With further development of suitable automated sampling techniques, it could become a suitable potential method for in-line quantification of maize endosperm contents. (C) 2004 Society of Chemical Industry.