Molecular characterization and functional distribution of N-ethylmaleimide-sensitive factor in Helicoverpa armigera

被引：23
作者：
Cui, Shuai-Ying
Xu, Wei-Hua [1 ]
机构：
[1] Univ Sci & Technol China, Sch Life Sci, Dept Mol & Cell Biol, Hefei 230027, Peoples R China
[2] Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
[3] Zhongshan Univ, Sch Life Sci, Inst Entomol, Guangzhou 510275, Peoples R China
来源：
PEPTIDES | 2006年 / 27卷 / 06期
基金：
中国国家自然科学基金;
关键词：
N-ethylmaleimide-sensitive fusion protein; neurosecretory cell; neurohormone release; fluorescence immunocytochemistry; co-localization; development;
D O I：
10.1016/j.peptides.2005.11.011
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
N-ethylmaleimide-sensitive fusion protein (NSF) is an essential component for the neurotransmitter or neurohormone release apparatus present in all eukaryotic cells. Here, a new NSF orthologue was characterized from the cotton bollworm, Helicoverpa armigera (Har). Northern blot exhibited a high expression in larval brain. Southern analysis indicated that a single copy of the gene is present in a haploid genome. Using antibodies labeled with fluoresceins, we directly proved that NSF is co-localized with two crucial neurohormones, prothoracicotropic hormone and diapause hormone, both of which regulate insect development. These findings suggest that Har-NSF may be involved in regulating insect neurohormone release. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：1226 / 1234
页数：9
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