Molecular characterization and functional distribution of N-ethylmaleimide-sensitive factor in Helicoverpa armigera

被引：23

作者：

Cui, Shuai-Ying

Xu, Wei-Hua ^{[1
]}

机构：

[1] Univ Sci & Technol China, Sch Life Sci, Dept Mol & Cell Biol, Hefei 230027, Peoples R China

[2] Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China

[3] Zhongshan Univ, Sch Life Sci, Inst Entomol, Guangzhou 510275, Peoples R China

来源：

PEPTIDES | 2006年 / 27卷 / 06期

基金：

中国国家自然科学基金;

关键词：

N-ethylmaleimide-sensitive fusion protein; neurosecretory cell; neurohormone release; fluorescence immunocytochemistry; co-localization; development;

D O I：

10.1016/j.peptides.2005.11.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-ethylmaleimide-sensitive fusion protein (NSF) is an essential component for the neurotransmitter or neurohormone release apparatus present in all eukaryotic cells. Here, a new NSF orthologue was characterized from the cotton bollworm, Helicoverpa armigera (Har). Northern blot exhibited a high expression in larval brain. Southern analysis indicated that a single copy of the gene is present in a haploid genome. Using antibodies labeled with fluoresceins, we directly proved that NSF is co-localized with two crucial neurohormones, prothoracicotropic hormone and diapause hormone, both of which regulate insect development. These findings suggest that Har-NSF may be involved in regulating insect neurohormone release. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1226 / 1234

页数：9

共 30 条

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