Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater

被引:30
|
作者
Dumke, Roger [1 ]
de la Cruz Barron, Magali [2 ,3 ]
Oertel, Reinhard [4 ]
Helm, Bjoern [5 ]
Kallies, Rene [6 ]
Berendonk, Thomas U. [2 ]
Dalpke, Alexander [1 ]
机构
[1] Tech Univ Dresden, Inst Med Microbiol & Virol, Fetscherstr 74, D-01307 Dresden, Germany
[2] Tech Univ Dresden, Inst Hydrobiol, Helmholtzstr 10, D-01069 Dresden, Germany
[3] UFZ Helmholtz Ctr Environm Res, Dept River Ecol, Bruckstr 3a, D-39114 Magdeburg, Germany
[4] Tech Univ Dresden, Inst Clin Pharmacol, Fetscherstr 74, D-01307 Dresden, Germany
[5] Tech Univ Dresden, Inst Urban & Ind Water Management, Helmholtzstr 10, D-01069 Dresden, Germany
[6] UFZ Helmholtz Ctr Environm Res, Dept Environm Microbiol, Permoserstr 15, D-04318 Leipzig, Germany
来源
PATHOGENS | 2021年 / 10卷 / 02期
关键词
SARS-CoV-2; wastewater; virus concentration; wastewater-based epidemiology; monitoring; surveillance; VIRUS; SURVEILLANCE;
D O I
10.3390/pathogens10020195
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
引用
收藏
页码:1 / 7
页数:7
相关论文
共 50 条
  • [41] Qualitative and quantitative detection of SARS-CoV-2 RNA in untreated wastewater in Western Cape Province, South Africa
    Johnson, R.
    Muller, C. J. F.
    Ghoor, S.
    Louw, J.
    Archer, E.
    Surujlal-Naicker, S.
    Berkowitz, N.
    Volschenk, M.
    Broecker, L. H. L.
    Wolfaardt, G.
    van der Walt, M.
    Mutshembele, A. M.
    Malema, S.
    Gelderblom, H. C.
    Muhdluli, M.
    Gray, G.
    Mathee, A.
    Street, R.
    SAMJ SOUTH AFRICAN MEDICAL JOURNAL, 2021, 111 (03): : 198 - 202
  • [42] Effect of Time and Temperature on SARS-CoV-2 in Municipal Wastewater Conveyance Systems
    Schussman, Melissa K.
    McLellan, Sandra L.
    WATER, 2022, 14 (09)
  • [43] Wastewater-based SARS-CoV-2 environmental monitoring for Piedmont, Italy
    Robotto, Angelo
    Lembo, David
    Quaglino, Paola
    Brizio, Enrico
    Polato, Denis
    Civra, Andrea
    Cusato, Jessica
    Di Perri, Giovanni
    ENVIRONMENTAL RESEARCH, 2022, 203 (203)
  • [44] Uncertainties in estimating SARS-CoV-2 prevalence by wastewater-based epidemiology
    Li, Xuan
    Zhang, Shuxin
    Shi, Jiahua
    Luby, Stephen P.
    Jiang, Guangming
    CHEMICAL ENGINEERING JOURNAL, 2021, 415 (415)
  • [45] Wastewater Surveillance for SARS-CoV-2 at Long-Term Care Facilities: Mixed Methods Evaluation
    Keck, James W.
    Lindner, Jess
    Liversedge, Matthew
    Mijatovic, Blazan
    Olsson, Cullen
    Strike, William
    Noble, Anni
    Adatorwovor, Reuben
    Lacy, Parker
    Smith, Ted
    Berry, Scott M.
    JMIR PUBLIC HEALTH AND SURVEILLANCE, 2023, 9 : e44657
  • [46] Defining biological and biophysical properties of SARS-CoV-2 genetic material in wastewater
    Robinson, Carolyn A.
    Hsieh, Hsin-Yeh
    Hsu, Shu-Yu
    Wang, Yang
    Salcedo, Braxton T.
    Belenchia, Anthony
    Klutts, Jessica
    Zemmer, Sally
    Reynolds, Melissa
    Semkiw, Elizabeth
    Foley, Trevor
    Wan, XiuFeng
    Wieberg, Chris G.
    Wenzel, Jeff
    Lin, Chung-Ho
    Johnson, Marc C.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 807
  • [47] Utilization of SARS-CoV-2 Wastewater Surveillance in Africa-A Rapid Review
    Dzinamarira, Tafadzwa
    Murewanhema, Grant
    Iradukunda, Patrick Gad
    Madziva, Roda
    Herrera, Helena
    Cuadros, Diego F.
    Tungwarara, Nigel
    Chitungo, Itai
    Musuka, Godfrey
    INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2022, 19 (02)
  • [48] SARS-CoV-2 existence in sewage and wastewater: A global public health concern?
    Dhama, Kuldeep
    Patel, Shailesh Kumar
    Yatoo, Mohd Iqbal
    Tiwari, Ruchi
    Sharun, Khan
    Dhama, Jaideep
    Natesan, Senthilkumar
    Malik, Yashpal Singh
    Singh, Karam Pal
    Harapan, Harapan
    JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2021, 280
  • [49] SARS-CoV-2 Monitoring in Wastewater Reveals Novel Variants and Biomarkers of Infection
    McGowan, Jenna
    Borucki, Monica
    Omairi, Hicham
    Varghese, Merina
    Vellani, Shahnaz
    Chakravarty, Sukanya
    Fan, Shumin
    Chattopadhyay, Srestha
    Siddiquee, Mashuk
    Thissen, James B.
    Mulakken, Nisha
    Moon, Joseph
    Kimbrel, Jeffrey
    Tiwari, Amit K.
    Taylor, Roger Travis
    Kang, Dae-Wook
    Jaing, Crystal
    Chakravarti, Ritu
    Chattopadhyay, Saurabh
    VIRUSES-BASEL, 2022, 14 (09):
  • [50] The first detection of SARS-CoV-2 RNA in urban wastewater in Giza, Egypt
    Shaheen, Mohamed
    Elmahdy, Elmahdy M.
    Shahein, Yasser E.
    JOURNAL OF WATER AND HEALTH, 2022, 20 (08) : 1212 - 1222