Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater

被引:30
|
作者
Dumke, Roger [1 ]
de la Cruz Barron, Magali [2 ,3 ]
Oertel, Reinhard [4 ]
Helm, Bjoern [5 ]
Kallies, Rene [6 ]
Berendonk, Thomas U. [2 ]
Dalpke, Alexander [1 ]
机构
[1] Tech Univ Dresden, Inst Med Microbiol & Virol, Fetscherstr 74, D-01307 Dresden, Germany
[2] Tech Univ Dresden, Inst Hydrobiol, Helmholtzstr 10, D-01069 Dresden, Germany
[3] UFZ Helmholtz Ctr Environm Res, Dept River Ecol, Bruckstr 3a, D-39114 Magdeburg, Germany
[4] Tech Univ Dresden, Inst Clin Pharmacol, Fetscherstr 74, D-01307 Dresden, Germany
[5] Tech Univ Dresden, Inst Urban & Ind Water Management, Helmholtzstr 10, D-01069 Dresden, Germany
[6] UFZ Helmholtz Ctr Environm Res, Dept Environm Microbiol, Permoserstr 15, D-04318 Leipzig, Germany
来源
PATHOGENS | 2021年 / 10卷 / 02期
关键词
SARS-CoV-2; wastewater; virus concentration; wastewater-based epidemiology; monitoring; surveillance; VIRUS; SURVEILLANCE;
D O I
10.3390/pathogens10020195
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
引用
收藏
页码:1 / 7
页数:7
相关论文
共 50 条
  • [31] A comparison of SARS-CoV-2 wastewater concentration methods for environmental surveillance
    Philo, Sarah E.
    Keim, Erika K.
    Swanstrom, Rachael
    Ong, Angelo Q. W.
    Burnor, Elisabeth A.
    Kossik, Alexandra L.
    Harrison, Joanna C.
    Demeke, Bethel A.
    Zhou, Nicolette A.
    Beck, Nicola K.
    Shirai, Jeffry H.
    Meschke, J. Scott
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 760
  • [32] Detection of SARS-CoV-2 RNA in wastewater and importance of population size assessment in smaller cities: An exploratory case study from two municipalities in Latvia
    Gudra, Dita
    Dejus, Sandis
    Bartkevics, Vadims
    Roga, Ance
    Kalnina, Ineta
    Strods, Martins
    Rayan, Anton
    Kokina, Kristina
    Zajakina, Anna
    Dumpis, Uga
    Ikkere, Laura Elina
    Arhipova, Irina
    Berzins, Gundars
    Erglis, Aldis
    Binde, Juris
    Ansonska, Evija
    Berzins, Aivars
    Juhna, Talis
    Fridmanis, Davids
    SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 823
  • [33] Wastewater-Based Epidemiology of SARS-CoV-2 RNA in Bethlehem, PA and Lehigh University
    Alqasawah, Nusaibah
    Ware, Vassie
    Jellison, Kristen
    ENVIRONMENTS, 2024, 11 (10)
  • [34] First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA
    Sherchan, Samendra P.
    Shahin, Shalina
    Ward, Lauren M.
    Tandukar, Sarmila
    Aw, Tiong G.
    Schmitz, Bradley
    Ahmed, Warish
    Kitajima, Masaaki
    SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 743
  • [35] Occurrence and transport of SARS-CoV-2 in wastewater streams and its detection and remediation by chemical-biological methods
    Bhattacharya, Sayan
    Abhishek, Kumar
    Samiksha, Shilpi
    Sharma, Prabhakar
    JOURNAL OF HAZARDOUS MATERIALS ADVANCES, 2023, 9
  • [36] Evaluation of SARS-CoV-2 RNA Presence in Treated and Untreated Hospital Sewage
    Qamsari, Elahe Mobarak
    Mohammadi, Parisa
    WATER AIR AND SOIL POLLUTION, 2023, 234 (04)
  • [37] Temporal dynamics of SARS-CoV-2 genome and detection of variants of concern in wastewater influent from two metropolitan areas in Arkansas
    Silva, Camila S.
    Tryndyak, Volodymyr P.
    Camacho, Luisa
    Orloff, Mohammed S.
    Porter, Austin
    Garner, Kelley
    Mullis, Lisa
    Azevedo, Marli
    SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 849
  • [38] High-throughput sequencing of SARS-CoV-2 in wastewater provides insights into circulating variants
    Fontenele, Rafaela S.
    Kraberger, Simona
    Hadfield, James
    Driver, Erin M.
    Bowes, Devin
    Holland, LaRinda A.
    Faleye, Temitope O. C.
    Adhikari, Sangeet
    Kumar, Rahul
    Inchausti, Rosa
    Holmes, Wydale K.
    Deitrick, Stephanie
    Brown, Philip
    Duty, Darrell
    Smith, Ted
    Bhatnagar, Aruni
    Yeager, Ray A., II
    Holm, Rochelle H.
    von Reitzenstein, Natalia Hoogesteijn
    Wheeler, Elliott
    Dixon, Kevin
    Constantine, Tim
    Wilson, Melissa A.
    Lim, Efrem S.
    Jiang, Xiaofang
    Halden, Rolf U.
    Scotch, Matthew
    Varsani, Arvind
    WATER RESEARCH, 2021, 205
  • [39] Detection of SARS-CoV-2 RNA in hospital wastewater from a low COVID-19 disease prevalence area
    Goncalves, Jose
    Koritnik, Tom
    Mioc, Verica
    Trkov, Marija
    Boljesic, Maja
    Berginc, Natasa
    Prosenc, Katarina
    Kotar, Tadeja
    Paragi, Metka
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 755
  • [40] Lessons learned from SARS-CoV-2 measurements in wastewater
    Sharkey, Mark E.
    Kumar, Naresh
    Mantero, Alejandro M. A.
    Babler, Kristina M.
    Boone, Melinda M.
    Cardentey, Yoslayma
    Cortizas, Elena M.
    Grills, George S.
    Herrin, James
    Kemper, Jenny M.
    Kenney, Richard
    Kobetz, Erin
    Laine, Jennifer
    Lamar, Walter E.
    Mader, Christopher C.
    Mason, Christopher E.
    Quintero, Anda Z.
    Reding, Brian D.
    Roca, Matthew A.
    Ryon, Krista
    Solle, Natasha Schaefer
    Schurer, Stephan C.
    Shukla, Bhavarth
    Stevenson, Mario
    Stone, Thomas
    Tallon, John J., Jr.
    Venkatapuram, Sreeharsha S.
    Vidovic, Dusica
    Williams, Sion L.
    Young, Benjamin
    Solo-Gabriele, Helena M.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 798 (798)