SimpleDSFviewer: A tool to analyze and view differential scanning fluorimetry data for characterizing protein thermal stability and interactions

被引：24

作者：

Sun, Changye ^{[1
,2
]}

Li, Yong ^{[2
]}

Yates, Edwin A. ^{[2
]}

Fernig, David G. ^{[2
]}

机构：

[1] Xinxiang Med Univ, Henan Key Lab Med Tissue Regenerat, Xinxiang 453003, Henan, Peoples R China

[2] Univ Liverpool, Inst Integrat Biol, Dept Biochem, Liverpool, Merseyside, England

来源：

PROTEIN SCIENCE | 2020年 / 29卷 / 01期

关键词：

data analysis; differential scanning fluorimetry; protein melting curve; protein melting temperature; PROMOTE;

D O I：

10.1002/pro.3703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential scanning fluorimetry (DSF) is a widely used thermal shift assay for measuring protein stability and protein-ligand interactions that are simple, cheap, and amenable to high throughput. However, data analysis remains a challenge, requiring improved methods. Here, the program SimpleDSFviewer, a user-friendly interface, is described to help the researchers who apply DSF technique in their studies. SimpleDSFviewer integrates melting curve (MC) normalization, smoothing, and melting temperature (Tm) analysis and directly previews analyzed data, providing an efficient analysis tool for DSF. SimpleDSFviewer is developed in Matlab, and it is freely available for all users to use in Matlab workspace or with Matlab Runtime. It is easy to use and an efficient tool for researchers to preview and analyze their data in a very short time.

引用

页码：19 / 27

页数：9

共 14 条

[11] STUDY OF THE MOLTEN GLOBULE INTERMEDIATE STATE IN PROTEIN FOLDING BY A HYDROPHOBIC FLUORESCENT-PROBE
SEMISOTNOV, GV
RODIONOVA, NA
RAZGULYAEV, OI
UVERSKY, VN
GRIPAS, AF
GILMANSHIN, RI
[J]. BIOPOLYMERS, 1991, 31 (01) : 119 - 128
[12] HaloTag is an effective expression and solubilisation fusion partner for a range of fibroblast growth factors
Sun, Changye
Li, Yong
Taylor, Sarah E.
Mao, Xianqing
Wilkinson, Mark C.
Fernig, David G.
[J]. PEERJ, 2015, 3
[13] Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Vivoli, Mirella
Novak, Halina R.
Littlechild, Jennifer A.
Harmer, Nicholas J.
[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (91):
[14] DMAN: a Java']Java tool for analysis of multi-well differential scanning fluorimetry experiments
Wang, Conan K.
Weeratunga, Saroja K.
Pacheco, Chris M.
Hofmann, Andreas
[J]. BIOINFORMATICS, 2012, 28 (03) : 439 - 440

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