Metabolic pathway for degradation of anthracene by halophilic Martelella sp AD-3

被引：28

作者：

Cui, Changzheng ^{[1
]}

Ma, Lei ^{[2
]}

Shi, Jie ^{[1
]}

Lin, Kuangfei ^{[1
]}

Luo, Qishi ^{[3
]}

Liu, Yongdi ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China

[2] E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

[3] Shanghai Acad Environm Sci, Inst Wastes & Soil Environm, State Environm Protect Engn Ctr Urban Soil Contam, Shanghai 200233, Peoples R China

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2014年 / 89卷

基金：

中国国家自然科学基金;

关键词：

Anthracene; Biodegradation; Moderate halophilic bacteria; Metabolic pathway; 3-Hydroxy-2-naphthoic acid; POLYCYCLIC AROMATIC-HYDROCARBONS; PHENANTHRENE; BIODEGRADATION; PURIFICATION;

D O I：

10.1016/j.ibiod.2014.01.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Anthracene (40 mg l(-1)) was completely depleted by Martelella sp. strain AD-3 under 3% salinity and a pH of 9.0 after 6 days of incubation. The metabolites were extracted and identified by high-performance liquid chromatography (HPLC) retention times, mass spectrometry, H-1 and C-13 nuclear magnetic resonance spectrometry, and comparison to authentic compounds or literature data. On the basis of the identified metabolites, enzyme activities and the utilization of probable intermediates, anthracene degradation by strain AD-3 is proposed via two distinct routes. In route I, metabolism of anthracene is initiated by the dioxygenation at C-1,2, then proceeds through 6,7-benzocoumarin, 3-hydroxy-2-naphthoic acid, salicylic acid and gentisic acid. In route II, anthracene is metabolized to 9,10-anthraquinone. The results suggest that strain AD-3 possesses efficient anthracene biodegradability in high salinity. To our knowledge, this work presents the first report of anthracene degradation by a halophilic PAH-degrading strain via two routes. The strain AD-3 may be a useful candidate for PAH-contaminated saline-alkali soil bioremediation. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：67 / 73

页数：7

共 29 条

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