Derivatization of steroids in biological samples for GC-MS and LC-MS analyses

被引：72

作者：

Marcos, Josep ^{[1
,2
]}

Pozo, Oscar J. ^{[3
]}

机构：

[1] Univ Pompeu Fabra, Dept Expt & Hlth Sci, Barcelona 08003, Spain

[2] LabcoDiagnostics, Dept Toxicol, Esplugas de Llobregat 08950, Spain

[3] Hosp del Mar, IMIM, Bioanal Res Grp, Barcelona 08003, Spain

来源：

BIOANALYSIS | 2015年 / 7卷 / 19期

关键词：

TANDEM MASS-SPECTROMETRY; DOPING-CONTROL ANALYSIS; GAS CHROMATOGRAPHY/MASS SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; MICROWAVE-ASSISTED DERIVATIZATION; CONGENITAL ADRENAL-HYPERPLASIA; PRESSURE CHEMICAL-IONIZATION; ANABOLIC-ANDROGENIC STEROIDS; DRIED BLOOD SPOTS; ELECTROSPRAY-IONIZATION;

D O I：

10.4155/bio.15.176

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of steroids in biological samples is essential in different areas of knowledge. MS combined with either GC or LC is considered the best analytical technique for specific and sensitive determinations. However, due to the physicochemical properties of some steroids, and the low concentrations found in biological samples, the formation of a derivative prior to their analysis is required. In GC-MS determinations, derivatization is needed for generating volatile and thermally stable compounds. The improvement in terms of stability and chromatographic retention are the main reasons for selecting the derivatization agent. On the other hand, derivatization is not compulsory in LC-MS analyses and the derivatization is typically used for improving the ionization and therefore the overall sensitivity achieved.

引用

页码：2515 / 2536

页数：22

共 149 条

[1]

Ackermans MT, 2014, BIOANALYSIS, V6, P43, DOI [10.4155/bio.13.300, 10.4155/BIO.13.300]

[2] The development of an optimized sample preparation for trace level detection of 17α-ethinylestradiol and estrone in whole fish tissue [J].