Co-Immobilization of Enzymes and Magnetic Nanoparticles by Metal-Nucleotide Hydrogelnanofibers for Improving Stability and Recycling

被引：38
作者：
Li, Chunfang [1 ]
Jiang, Shuhui [2 ]
Zhao, Xinying [3 ]
Liang, Hao [2 ]
机构：
[1] Beijing Ind Tech Coll, Dept Environm Protect & Detect, Beijing 100023, Peoples R China
[2] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
[3] Beijing Ctr Phys & Chem Anal, Beijing 100089, Peoples R China
来源：
MOLECULES | 2017年 / 22卷 / 01期
基金：
国家高技术研究发展计划(863计划); 北京市自然科学基金;
关键词：
metal; nucleotide; magnetic iron oxide nanoparticles; Candida rugose lipase (CRL); immobilized enzyme; PORCINE PANCREATIC LIPASE; FE3O4; NANOPARTICLES; IMMOBILIZED LIPASE; EFFICIENT IMMOBILIZATION; RECOVERABLE BIOCATALYST; ORGANIC FRAMEWORKS; PERFORMANCE; SUPPORT; CARRIER; MICROSPHERES;
D O I：
10.3390/molecules22010179
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers. Candida rugosa lipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe3O4 NPs) have been widely used for immobilizing enzymes. As a result, the relative activity of CA-Fe3O4@Zn/AMP nanofiber-immobilized CRL increased by 8-fold at pH 10.0 and nearly 1-fold in a 50 degrees C water bath after 30 min, compared to free CRL. Moreover, the immobilized CRL had excellent long-term storage stability (nearly 80% releative activity after storage for 13 days). This work indicated that metal-nucleotide nanofibers could efficiently co-immobilize enzymes and MNPs simultaneously, and improve the stability of biocatalysts.
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页数：11
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