Behaviour of beta(2)-microglobulin in the electrical double layer of the mercury vertical bar solution interface

被引:4
作者
Vidic, J
Suznjevic, D
Erceg, M
Vucelic, D
机构
[1] FORENS INST, YU-11000 BELGRADE, YUGOSLAVIA
[2] UNIV BELGRADE, FAC PHYS CHEM, YU-11000 BELGRADE, YUGOSLAVIA
来源
BIOELECTROCHEMISTRY AND BIOENERGETICS | 1996年 / 39卷 / 01期
关键词
alternating current polarography; beta(2)-microglobulin; adsorption; conformation; mercury electrode;
D O I
10.1016/0302-4598(95)01874-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
beta(2)-Microglobulin (beta(2)-m) is a small globular protein (12000 Da) which exists as a monomer in numerous body fluids. Data published on the electrochemical behaviour of beta(2)-m are scarce. In this paper an attempt was made to get more information on the structure of the double layer at mercury/solution interface in the presence of beta(2)-m. Using phase selective ac polarography the capacitive current-potential (C-E) curve was analysed as the function of various beta(2)-m concentration, pH of medium, temperature, denaturation agent concentration etc. The data obtained are discussed particularly from the aspect of folding/unfolding state of the protein studied.
引用
收藏
页码:129 / 134
页数:6
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