Comparison of HbA1c detection in whole blood and dried blood spots using an automated ion-exchange HPLC system

被引：18

作者：

Maleska, Aleksandra ^{[1
,2
,3
]}

Hirtz, Christophe ^{[1
]}

Casteleyn, Elise ^{[1
]}

Villard, Orianne ^{[4
]}

Ducos, Jacques ^{[2
,3
]}

Avignon, Antoine ^{[4
]}

Sultan, Ariane ^{[4
]}

Lehmann, Sylvain ^{[1
]}

机构：

[1] Univ Montpellier, CHU Montpellier, INSERM U1183, Lab Biochim Proteom Clin, F-34000 Montpellier, France

[2] CHU Montpellier, Hop Lapeyronie, Lab Virol, F-34000 Montpellier, France

[3] INSERM, U1058, F-34000 Montpellier, France

[4] Univ Montpellier, Univ Hosp, CNRS UMR 9214, PhyMedExp,INSERM U1046,Endocrinol Diabetol Nutr D, Montpellier, France

来源：

BIOANALYSIS | 2017年 / 9卷 / 05期

关键词：

clinical biology; DBS; dried blood spot; glycated hemoglobin; HbA1c; HPLC; ion exchange; ISO15189; method validation; DIABETES CONTROL; COMPLICATIONS TRIAL/EPIDEMIOLOGY; HEMOGLOBIN A1C; FILTER-PAPER; INTERVENTIONS; OUTCOMES; GLUCOSE; PATIENT; HBA(1C); SAMPLES;

D O I：

10.4155/bio-2016-0278

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: Hemoglobin A1c (HbA1c) is a widely recognized analyte for diagnosing and monitoring diabetes. Dried blood spot (DBS) constitutes a useful alternative to blood collection by venipuncture. Analytical and clinical validation of DBS use is, however, necessary before implementation. Results/methodology: HbA1c levels from whole blood or DBS from a cohort patients with diabetes were compared. DBS specimens were stable at ambient temperature. HbA1c detection on DBS was accurate, robust, and the correlation and agreement with whole blood values was excellent. Conclusion: This study provides for the first time a complete method comparison and validation under the ISO15189 guideline using an automated HPLC system. This approach constitutes, therefore, a useful tool for diagnosing diabetes.

引用

页码：427 / 434

页数：8

共 26 条

[1] Comparability of HbA1c and lipids measured with dried blood spot versus venous samples: a systematic review and meta-analysis
Affan, Eshan T.
Praveen, Devarsetty
Chow, Clara K.
Neal, Bruce C.
[J]. BMC CLINICAL PATHOLOGY, 2014, 14
[2] Executive Summary: Standards of Medical Care in Diabetes-2014
不详
[J]. DIABETES CARE, 2014, 37 : S5 - S13
[3] [Anonymous], SURVEY OPHTHALMOLOGY
[4] STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT
BLAND, JM
ALTMAN, DG
[J]. LANCET, 1986, 1 (8476) : 307 - 310
[5] Treatment of the patient with diabetes:: importance of maintaining target HbA1c levels
Davidson, JA
[J]. CURRENT MEDICAL RESEARCH AND OPINION, 2004, 20 (12) : 1919 - 1927
[6] Albuminuria Changes and Cardiovascular and Renal Outcomes in Type 1 Diabetes: The DCCT/EDIC Study
de Boer, Ian H.
Gao, Xiaoyu
Cleary, Patricia A.
Bebu, Ionut
Lachin, John M.
Molitch, Mark E.
Orchard, Trevor
Paterson, Andrew D.
Perkins, Bruce A.
Steffes, Michael W.
Zinman, Bernard
[J]. CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2016, 11 (11): : 1969 - 1977
[7] Dewitte K, 2002, CLIN CHEM, V48, P799
[8] Measurement of Hemoglobin A1c from Filter Papers for Population-Based Studies
Egier, David A.
Keys, Judy L.
Hall, S. Kim
McQueen, Matthew J.
[J]. CLINICAL CHEMISTRY, 2011, 57 (04) : 577 - 585
[9] HbA1c measurements from dried blood spots: validation and patient satisfaction
Fokkema, M. Rebecca
Bakker, Andries J.
de Boer, Fokje
Kooistra, Jeltsje
de Vries, Sifra
Wolthuis, Albert
[J]. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2009, 47 (10) : 1259 - 1264
[10] ACCURACY OF A FILTER-PAPER METHOD FOR MEASURING GLYCOSYLATED HEMOGLOBIN
GAY, EC
CRUICKSHANKS, KJ
CHASE, HP
KLINGENSMITH, G
HAMMAN, RF
[J]. DIABETES CARE, 1992, 15 (01) : 108 - 110

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