Simultaneous overexpression of multidrug efflux pumps in Pseudomonas aeruginosa non-cystic fibrosis clinical isolates

被引:26
作者
Poonsuk, Kanchana [1 ,2 ]
Tribuddharat, Chanwit [3 ]
Chuanchuen, Rungtip [1 ]
机构
[1] Chulalongkorn Univ, Fac Vet Sci, Dept Vet Publ Hlth, Res Unit Microbial Food Safety & Antimicrobial Re, Bangkok, Thailand
[2] Chulalongkorn Univ, Fac Vet Sci, Bangkok, Thailand
[3] Mahidol Univ, Fac Med, Dept Microbiol, Bangkok 10700, Thailand
关键词
multidrug resistance; multidrug efflux system; Pseudomonas aeruginosa; MEXAB-OPRM; MEXEF-OPRN; AMINOGLYCOSIDE RESISTANCE; INTRINSIC RESISTANCE; MEXCD-OPRJ; EXPRESSION; SYSTEM; GENE; MUTATIONS; REPRESSOR;
D O I
10.1139/cjm-2014-0239
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically important antibiotics. Some isolates simultaneously overexpressed up to 4 different Mex systems, as determined by quantitative real-time reverse transcription PCR. None of the isolates overexpressed MexCD-OprJ, and only 1 isolate overproduced MexJK. All the isolates overexpressed MexXY, while overexpression of MexEF-OprN and MexVW was common. DNA sequencing analysis of regulatory genes showed that no clear correlation could be established among (i) the presence of mutations, (ii) the type of mutations, (iii) the expression level of the Mex systems, and (iv) resistance to antibiotic substrates. The results suggest that the concomitant overexpression of some Mex systems may superimpose their antimicrobial drug efflux capabilities, contributing to the multidrug resistance phenotype in the P. aeruginosa non-CF clinical isolates. The existence of uncharacterized regulators for the Mex systems was signified.
引用
收藏
页码:437 / 443
页数:7
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