Sequence selective dual-emission detection of (i, i+1) bis-phosphorylated peptide using diazastilbene-type Zn(II)-Dpa chemosensor

被引:32
作者
Ishida, Yoshiyuki [1 ]
Inoue, Masa-aki [1 ]
Inoue, Tomonori [1 ]
Ojida, Akio [1 ]
Hamachi, Itaru [1 ]
机构
[1] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Kyoto 6158510, Japan
关键词
MOLECULAR RECOGNITION; ARTIFICIAL RECEPTORS; AQUEOUS-SOLUTION; DERIVATIVES;
D O I
10.1039/b905814a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
This paper describes a new fluorescent chemosensor for phosphorylated peptide, which comprises a rigid trans-4,4'-diazastilbene and two Zn(II)-Dpa (2,2'-dipicolylamine) units; this chemosensor sequence-selectively binds to a (i, i + 1) bis-phosphorylated peptide and displays a dual-emission fluorescence change.
引用
收藏
页码:2848 / 2850
页数:3
相关论文
共 12 条
[1]   Synthetic Receptors for the Differentiation of Phosphorylated Peptides with Nanomolar Affinities [J].
Grauer, Andreas ;
Riechers, Alexander ;
Ritter, Stefan ;
Koenig, Burkhard .
CHEMISTRY-A EUROPEAN JOURNAL, 2008, 14 (29) :8922-8927
[2]   Kinomics: methods for deciphering the kinome [J].
Johnson, SA ;
Hunter, T .
NATURE METHODS, 2005, 2 (01) :17-25
[3]   Phosphate-binding tag, a new tool to visualize phosphorylated proteins [J].
Kinoshita, E ;
Kinoshita-Kikuta, E ;
Takiyama, K ;
Koike, T .
MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (04) :749-757
[4]   Effective disruption of phosphoprotein-protein surface interaction using Zn(II) dipicolylamine-based artificial receptors via two-point interaction [J].
Ojida, A ;
Inoue, M ;
Mito-oka, Y ;
Tsutsumi, H ;
Sada, K ;
Hamachi, I .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (06) :2052-2058
[5]   Molecular recognition and fluorescence sensing of monophosphorylated peptides in aqueous solution by bis(zinc(II)-dipicolylamine)-based artificial receptors [J].
Ojida, A ;
Mito-oka, Y ;
Sada, K ;
Hamachi, I .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (08) :2454-2463
[6]   Cross-linking strategy for molecular recognition and fluorescent sensing of a multi-phosphorylated peptide in aqueous solution [J].
Ojida, A ;
Inoue, M ;
Mito-oka, Y ;
Hamachi, I .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (34) :10184-10185
[7]  
OJIDA A, 2004, CHEM LETT, V33
[8]   FLUORESCENCE LIFETIMES OF SEVERAL STRUCTURALLY RIGID T-STILBENE DERIVATIVES [J].
PYUN, CH ;
LYLE, TA ;
DAUB, GH ;
PARK, SM .
CHEMICAL PHYSICS LETTERS, 1986, 124 (01) :48-52
[9]   Chemical approaches for investigating phosphorylation in signal transduction networks [J].
Rothman, DM ;
Shults, MD ;
Imperiali, B .
TRENDS IN CELL BIOLOGY, 2005, 15 (09) :502-510
[10]   Molecular recognition, fluorescence sensing, and biological assay of phosphate anion derivatives using artificial Zn(II)-Dpa complexes [J].
Sakamoto, Takashi ;
Ojida, Akio ;
Hamachi, Itaru .
CHEMICAL COMMUNICATIONS, 2009, (02) :141-152